虾副产物自溶过程研究与潜在抗冻肽鉴定  

Autolysis Process of Shrimp By-products and Identification of Potential Antifreeze Peptides

作　　者：尹雪莲 白雪 朱凯 戴志远 YIN Xuelian;BAI Xue;ZHU Kai;DAI Zhiyuan(Institute of Seafood,Zhejiang Gongshang University,Hangzhou 310012,China;Key Laboratory of Aquatic Products Processing of Zhejiang Province,Hangzhou 310012,China)

机构地区：[1]浙江工商大学海洋食品研究院,浙江杭州310012 [2]浙江省水产品加工技术研究联合重点实验室,浙江杭州310012

出　　处：《食品工业科技》2023年第6期33-40,共8页Science and Technology of Food Industry

基　　金：“蓝色粮仓”国家重点研发计划项目(2019YFD0902000)。

摘　　要：为加强海洋副产物的高值化加工利用,本文以虾副产物为原料,在原始pH(7.81~7.83)与50℃水浴条件下进行自溶反应,将酶解产物于95℃水浴加热20 min使内源酶失活,研究了过程中酶解产物的多肽组成变化,并基于活性预测平台筛选得到抗冻肽序列,提供了一种高效率筛选活性肽的方法。自溶处理的0~3 h,酶解物的水解度(DH)和TCA可溶性肽含量急剧增加,分别达到49.8%和26.1 mg/g,随后增长速度变缓。在酶解0.5 h产物(A-0.5)中检出1301条肽段,酶解3 h产物(A-3)中检出197条;A-0.5中大于1600 Da的肽含量达到69.5%,而A-3中肽以600~1600 Da为主。基于活性预测平台Cryoprotect,筛选QVHPDTGISS、GYGCARPNYPGV、TTGEVCDSGDGVTH、EQICINFCNEK、DEYEESGPGIVH、DIDNDGFLDK和DIDNNGFLDK 7个序列为抗冻肽,并通过酶切模拟得到肽段的来源。In order to strengthen the high-value processing and utilization of marine by-products, shrimp by-products were used in this study. The autolysis reaction was carried out in a water bath at 50 ℃ and pH(7.81~7.83), then the enzymatic hydrolysate was heated in water bath at 95 ℃ for 20 min to inactivate endogenous enzymes. This paper studied the changes in the polypeptide composition of the enzymatic hydrolysis products during the process, and screened the antifreeze peptide sequences based on the activity prediction platform, and provided a method for screening active peptides with high efficiency. When the autolysis treatment was 0~3 h, the content of degree of hydrolysis(DH) and TCA soluble peptides in the enzymatic hydrolyzate increased sharply, reaching 49.8% and 26.1 mg/g, respectively, and then the growth rate slowed down. 1301 peptides were detected in the 0.5 h enzymatic hydrolysis product(A-0.5), and 197 in the 3 h enzymatic hydrolysis product(A-3). The content of peptides greater than 1600 Da in A-0.5 reached 69.5%, while the peptides in A-3were dominated by 600~1600 Da. Based on the activity prediction platform Cryoprotect, 7 sequences of QVHPDTGISS,GYGCARPNYPGV, TTGEVCDSGDGVTH, EQICINFCNEK, DEYEESGPGIVH, DIDNDGFLDK and DIDNNGFLDK were screened as antifreeze peptides, and the source of the peptides was obtained by enzyme cleavage simulation.

关 键 词：自溶 多肽组成 活性预测 抗冻肽 虾副产物 

分 类 号：S985.21[农业科学—捕捞与储运]