邻苯二酚对酿酒酵母理化特性的影响  

Effects of Catechol on Physicochemical Properties of Saccharomyces cerevisiae

作　　者：黄锦翔 易弋[1] 梁君夏 丰丕雪 马振花 严寅珲 龙秀锋 Huang Jinxiang;Yi Yi;Liang Junxia;Feng Pixue;Ma Zhenhua;Yan Yinhui;Long Xiufeng(College of Biological and Chemical Engineering,Guangxi University of Science and Technology,Guangxi Key Laboratory of Green Processing of Sugar Resources,Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes,Liuzhou 545006,Guangxi)

机构地区：[1]广西科技大学生物与化学工程学院、广西糖资源绿色加工重点实验室、广西高校糖资源加工重点实验室,广西柳州545006

出　　处：《中国食品学报》2023年第3期130-137,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31560728);广西自然科学基金项目(2018GXNSFAA050116)。

摘　　要：邻苯二酚是木质纤维素水解液中一种主要的细胞生长抑制物,毒性作用较强,能抑制后续酒精发酵过程中酿酒酵母的生长和发酵性能。本文研究邻苯二酚对酿酒酵母GGSF16理化特性的影响,结果表明,邻苯二酚质量浓度为1.2 g/L时,明显抑制酿酒酵母细胞的生长和代谢,导致消耗葡萄糖的时间变慢和乙醇发酵周期变长,降低乙醇生成率;明显改变酵母细胞的膜通透性,使胞内核酸和蛋白质发生内漏,含量分别是对照组的2.50,2.01倍;细胞内的应激代谢物海藻糖含量显著升高2.89倍;此外,为了抵抗细胞的氧化损伤,胞内GSH含量显著降低70.63%。扫描电子显微镜显示:用邻苯二酚处理后,表面光滑、饱满的酵母细胞的细胞壁和细胞膜被破坏,出现空洞、大量撕裂碎片和彼此之间粘黏等现象,细胞失去保护作用而死亡。本研究初步明确了酚类物质对酿酒酵母的损伤和毒性机制,为进一步揭示木质纤维素水解液对酿酒酵母的抑制作用提供参考。Catechol is a major cell growth inhibitor in lignocellulose hydrolysate,with strong toxicity,and can inhibit the growth and fermentation performance of Saccharomyces cerevisiae during subsequent alcohol fermentation.The effects of catechol on the physical and chemical properties of Saccharomyces cerevisiae GGSF 16 were studied.The results showed that when the concentration of catechol was 1.2 g/L,the growth and metabolism of Saccharomyces cerevisiae cells were significantly inhibited,resulting in slower glucose consumption time and longer ethanol fermentation cycle,and lower ethanol production rate.The membrane permeability of yeast cells was significantly changed,and the content of nucleic acid and protein in the cells was 2.50 and 2.01 times higher than that in the control group.The content of trehalose,a stress metabolite in cells,increased significantly by 2.89 times.In addition,in order to resist the oxidative damage of cells,the content of intracellular GSH decreased significantly by 70.63%.Scanning electron microscope showed that after treatment with catechol,the cell wall and cell membrane of smooth and full yeast cells were damaged,and there were cavities,a large number of torn fragments and adhesion between them.The cells lost their protective effect and died.This study preliminarily clarified the damage and toxicity mechanism of phenols on Saccharomyces cerevisiae,and provided a reference for further revealing the inhibitory effect of lignocellulose hydrolysate on Saccharomyces cerevisiae.

关 键 词：邻苯二酚 酿酒酵母 理化特性 谷胱甘肽 氧化胁迫 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]