pmrA影响酸胁迫鼠伤寒沙门氏菌生物膜的形成能力  

The Effect of pmrA on Biofilm Formation Ability of Acid Stressed Salmonella typhimurium

作　　者：杨克慧 董鹏程[1] 刘昀阁 张一敏[1] 毛衍伟[1] 梁荣蓉[1] 罗欣[1] 朱立贤[1] Yang Kehui;Dong Pengcheng;Liu Yunge;Zhang Yimin;Mao Yanwei;Liang Rongrong;Luo Xin;Zhu Lixian(College of Food Science and Engineering,Shandong Agricultural University,Taian 271018,Shandong)

机构地区：[1]山东农业大学食品科学与工程学院,山东泰安271018

出　　处：《中国食品学报》2023年第3期147-156,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：山东省现代农业产业技术体系创新团队建设专项(SDAIT-09-09);现代农业产业技术体系建设专项资金资助-肉牛项目(CARS-37);国家自然科学基金青年科学基金项目(31801609);国家重点研发政府间国际科技创新合作专项(2019YFE0103800)。

摘　　要：为探究pmrA基因对酸胁迫鼠伤寒沙门氏菌生物膜形成能力的影响,以鼠伤寒沙门氏菌野生株(WT)和pmrA基因缺失株(ΔpmrA)为研究对象,对其诱导耐酸能力、菌株特性、生物膜形成能力以及影响生物膜形成的内在因素进行研究。结果:pH 5.4胁迫后ΔpmrA的诱导耐酸能力为WT的53.92%;生物膜培养至第4天,经酸胁迫(pH 5.4)后ΔpmrA的生物膜形成量仅为WT的54.68%;经酸胁迫后,ΔpmrA的泳动能力、疏水性分别为WT的35.20%,59.10%;ΔpmrA经酸胁迫后其生物膜代谢活性、胞外多糖和蛋白的生物合成量与未酸胁迫处理相比虽有一定程度升高,但仍显著低于酸胁迫后的WT(P<0.05),同时酸胁迫后pmrA基因缺失株生物膜的三维立体结构仍较为分散,且膜内活细胞数量显著低于WT。结论:pmrA基因与鼠伤寒沙门氏菌的耐酸能力及生物膜形成能力密切相关。In order to explore the role of pmrA gene in acid tolerance resistance and biofilm formation of Salmonella typhimurium(S.typhimurium)induced after acid stress.Measuring the growth curve,acid tolerance response,and biofilm formation ability of the wild-type(WT)strain of S.typhimurium and pmrA deletion mutant strain(ΔpmrA)to preliminarily confirm whether acid stress and the lack of pmrA affect the biofilm formation ability of S.typhimurium.Further determined the strain characteristics of WT andΔpmrA such as swimming ability,surface hydrophobic ability and self-aggregation.At the same time,metabolic activity,extracellular polymeric substance(EPS)content and biofilm microstructure of the biofilm formation also should be analyzed to explore the mechanism of pmrA deletion on the biofilm formation ability of acid stressed S.typhimurium.The result:After acid stress,the acid tolerance ofΔpmrA was 53.92%of WT.On the 4th day of biofilm culture,the biofilm formation ofΔpmrA was only 54.68%of WT.The swimming ability and hydrophobicity ofΔpmrA were 35.20%and 59.10%of WT,respectively.After acid stress,the biofilm metabolic activity and the extracellular polymeric substances content ofΔpmrA were increased to a certain extent compared with those which had not experienced,however,they were dramatically lower than those of the WT strain(P<0.05).In the meantime,the three-dimensional structure of the biofilm of theΔpmrA was still relatively scattered and the number of living cells in the membrane were prominently less than the WT strain(P<0.05).Conclusion:pmrA gene was closely associated with S.Typhimurium,especially in acid tolerance response and biofilm formation.

关 键 词：沙门氏菌 pmrA基因 酸胁迫 生物膜 耐酸性 菌株特性 

分 类 号：TS201.3[轻工技术与工程—食品科学]