基于斑马鱼模型评价白茶的降糖作用  

Hypoglycemic Effects of White Tea Based on the Zebrafish Model

作　　者：刘均[1] 李强[1] 谭蓉[1] LIU Jun;LI Qiang;TAN Rong(Hangzhou Tea Research Institute,CHINA COOP,Zhejiang Key Laboratory of Transboundary Applied Technology for Tea Resources,Hangzhou 310016,China)

机构地区：[1]中华全国供销合作总社杭州茶叶研究院,浙江省茶资源跨界应用技术重点实验室,浙江杭州310016

出　　处：《现代食品科技》2023年第3期45-54,共10页Modern Food Science and Technology

基　　金：浙江省公益基础研究农业项目(LGN18C200010)。

摘　　要：研究以白茶为对象,采用新型斑马鱼实验技术开展了白茶降糖比较研究。基于非胰岛素依赖型糖尿病(NIDDM)斑马鱼研究发现,50和100μg/mL老白牡丹茶(AWP)孵育24 h葡萄糖(GLU)值水平分别降低了29.68%和20.51%,孵育48 h GLU值分别降低了42.16%和51.54%,差异均显著(P<0.05);25、50和75μg/mL白牡丹茶(WP)孵育24 h GLU值分别降低了40.18%、52.57%和62.11%,差异均显著(P<0.05)。另外,基于胰岛素依赖型糖尿病(IDDM)斑马鱼研究,20和30μg/mL AWP孵育24 h GLU值分别降低了24.92%和23.66%,孵育48 h分别降低了54.35%和38.38%,差异均显著(P<0.05);40μg/mL WP孵育24 h GLU值降低了22.58%(P<0.05),20μg/mL WP孵育48 h降低了26.51%(P<0.05);30μg/mL茶多酚(TP)孵育48 h GLU值降低了24.53%(P<0.05)。重要的是,在同等生药量下,20μg/mL WP和AWP对NIDDM斑马鱼的降糖效果显著优于TP;30μg/mL AWP对IDDM斑马鱼的降糖效果略优于TP,再明显优于WP。结果表明白茶具有明显的降糖功效,TP也具有明确的降糖作用,但白茶的降糖效应并不仅限于依赖TP,具体何成分起关键作用尚值得关注和研究。The hypoglycemic effects of white tea were investigated,using a newly developed non-insulin-dependent diabetes mellitus(NIDDM)zebrafish model..Treatment of the NIDDM zebra fish with 50 and 100μg/mL of aged white peony tea(AWP),decreased the level of glucose(GLU)by 29.68%and 20.51%,respectively,after 24 h.After 48 h,the GLU values decreased by 42.16%and 51.54%,respectively,and the differences were significant(P<0.05).Moreover,the GLU values in the 25,50,and 75μg/mL white peony tea(WP)groups decreased by 40.18%,52.57%,and 62.11%,respectively,after 24 h of treatment,and the differences were significant(P<0.05).The effect of white tea treatment on GLU levels of insulin-dependent diabetes mellitus(IDDM)zebrafish was also investigated.In this model,the GLU values of the 20 and 30μg/mL AWP treatment groups(24 h)decreased by 24.92%and 23.66%,respectively,and AWP treatment for 48 h decreased GLU by 54.35%and 38.38%,respectively(P<0.05).The GLU value of the 40μg/mL WP-treated group decreased by 22.58%(P<0.05)after 24 h,and the corresponding values in the 20μg/mL WP-treated group,after 48 h of treatment,decreased by 26.51%(P<0.05).After incubation with 30μg/mL tea polyphenols(TP)for 48 h,the GLU value decreased by 24.53%(P<0.05).Importantly,the hypoglycemic effects of 20μg/mL of either WP and AWP on NIDDM zebrafish were significantly better than that of 30μg/mL of tea phenols(TP).The hypoglycemic effect of 30μg/mL AWP on IDDM zebrafish was slightly better than that of TP,and significantly better than that of WP.Taken together,these results suggest that both white tea and TP have a significant hypoglycemic effect;however,the hypoglycemic effect of white tea was not limited to TP dependence.The identity of the specific active components in white tea warrant further research.

关 键 词：斑马鱼 糖尿病 白茶 降糖作用 耐受性 茶多酚 

分 类 号：TS272.5[农业科学—茶叶生产加工] TS201.4[轻工技术与工程—农产品加工及贮藏工程]