高效液相色谱法测定食用植物油中黄曲霉毒素B_(1)前处理方法的改进  

Improvement of pretreatment method for determination of aflatoxin B_(1)in edible vegetable oil by HPLC

作　　者：蒋志维 覃洁 陈谢平[2] 莫素青 JIANG Zhi-wei;QIN Jie;CHEN Xie-ping;MO Su-qing(Guangxi Zhuang Autonomous Region Light Industry Product Quality Inspection Station,Nanning 530031,Guangxi,China;Guangxi Nanning Chemical Pharmaceutical Co.,Ltd.,Nanning 530031,Guangxi,China)

机构地区：[1]广西壮族自治区轻工产品质量检验站,广西南宁530031 [2]广西南宁化学制药有限责任公司,广西南宁530031

出　　处：《粮食与油脂》2023年第4期160-162,共3页Cereals & Oils

摘　　要：改进GB 5009.22—2016《食品安全国家标准食品中黄曲霉毒素B族和G族的测定》中高效液相色谱法测定食用植物油中的黄曲霉毒素B_(1)的方法,将甲醇-水溶液(体积比70∶30)用量提高至25 mL,选择均质提取4 min作为前处理提取方式,加入5.0 g氯化钠消除乳化现象,取消氮吹过程,净化洗脱完后直接定容。该优化方法提取效率高,较好地减少了被检测含量的损失,净化过程较原有方法更简单、方便,回收率和精密度更满意,适用于植物油脂黄曲霉毒素B_(1)的检测分析。The method for determining aflatoxin B_(1)in edible vegetable oil by high performance liquid chromatography in GB 5009.22-2016 National Standard for Food Safety-Determination of aflatoxin B and G groups was optimized.The amount of methanol-water solution(volume ratio 70:30)was increased to 25 mL,homogeneous extraction for 4 min was selected as the pretreatment extraction method,5.0 g sodium chloride was added to eliminate emulsification,the nitrogen blowing process was canceled,and the volume was directly fixed after purification and elution.The optimized method had high extraction ef­ficiency,and could reduce the loss of the detected content well.The purification process was simpler and more convenient than the original method.The recovery rate and precision were more satisfactory.It was suitable for the detection and analysis of aflatoxin B_(1)in vegetable oil.

关 键 词：高效液相色谱法 食用植物油 黄曲霉毒素B_(1) 前处理 改进 

分 类 号：TS207.5[轻工技术与工程—食品科学]