基于多指标分析优化苦丁茶冬青瞬时高温灭菌工艺  

Optimize the Instantaneous High Temperature Sterilization Process of Ilex kudingcha Based on Multiple Index Analysis

作　　者：张婷 焦连庆[2] 刘融融[2,3] 于敏 梁善芳[4] 张凤清 ZHANG Ting;JIAO Lianqing;LIU Rongrong;YU Min;LIANG Shanfang;ZHANG Fengqing(College of Chemistry and Life Sciences,Changchun University of Technology,Changchun 130102,China;Jinlin Provincial Academy of Traditional Chinese Medicine,Changchun 130012,China;College of Chiese Medicinal Material,Jilin Agricultural University,Changchun 130118,China;Beijing Baode Runsheng Health Management Co.,Ltd.,Beijing 102200,China)

机构地区：[1]长春工业大学化学与生命科学学院,吉林长春130102 [2]吉林省中医药科学院,吉林长春130012 [3]吉林农业大学中药材学院,吉林长春130118 [4]北京宝德润生健康管理有限公司,北京102200

出　　处：《食品工业科技》2023年第8期205-211,共7页Science and Technology of Food Industry

基　　金：吉林省科技发展计划项目(20200404092YY);吉林省发展和改革委员会(2020C032-6)。

摘　　要：基于多指标优化苦丁茶冬青瞬时高温灭菌工艺参数。采用正交设计多指标综合加权评分法优化苦丁茶冬青瞬时高温灭菌工艺参数,以灭菌温度、灭菌时间、药材粒度为考察因素,以灭菌率、1,1-二苯基-2-苦基肼(DPPH·)抗氧化活性IC_(50)值、多酚类化合物含量为考察指标,采用直观及方差分析结果评价灭菌对3个考察指标的影响;采用指纹图谱技术对灭菌前后的样品进行评价。结果表明,灭菌温度是具有显著影响的因素(P<0.05),确定的最佳灭菌工艺条件为:灭菌温度160℃,灭菌时间10 s,粉碎粒度24目。瞬时高温灭菌对DPPH·抗氧化活性IC_(50)值以及多酚类化合物含量均无显著影响(P>0.05)。按优化工艺灭菌后的3批样品微生物限度检查均符合药典规定;灭菌后3批样品的DPPH·抗氧化活性IC_(50)值较灭菌前减少0.19,多酚类化合物含量较灭菌前增加约0.14%;灭菌前后3批样品的DPPH·抗氧化活性IC_(50)值及多酚类化合物含量结果经t检验均无显著差异(P>0.05);灭菌前后样品指纹图谱相似度>0.9。综上所述,瞬时高温灭菌对苦丁茶冬青质量无影响,为瞬时高温灭菌技术在苦丁茶冬青灭菌中的应用提供了实验依据。To optimize the instantaneous high-temperature sterilization process parameters of Ilex kudingcha based on multiple indicators.The process parameters of instantaneous high-temperature sterilization of Ilex kudingcha were optimized by using the orthogonal design multi-index comprehensive weighted scoring method.Sterilization temperature,sterilization time,and particle size of herbs were used as the investigating factors,the sterilization rate,IC_(50)value of antioxidant activity of 1,1-diphenyl-2-picrylhydrazyl(DPPH·),and polyphenolic content were used as indicators,visual and ANOVA results were used to evaluate the effect of sterilization on the three examined indicators.Fingerprint technology was used to evaluate the samples before and after sterilization.The results showed that sterilization temperature was a factor that had a significant impact(P<0.05),and the optimal sterilization process conditions were as follows:Sterilization temperature 160℃,sterilization time 10 s,grinding size 24 mesh.No significant effect of instantaneous high-temperature sterilization on IC_(50)values of DPPH·antioxidant activity and content of polyphenolic compounds(P>0.05).The microbiological limits of the three batches of samples sterilized by the optimized process were all in accordance with the provisions of pharmacopoeia.The IC_(50)values of DPPH·antioxidant activity of the three batches of samples after sterilization decreased by 0.19 and the polyphenolic content increased by about 0.14%compared to that before sterilization.The results of DPPH·antioxidant activity IC_(50)values and polyphenolic compounds content of the three batches of samples before and after sterilization were not significantly different by t-test(P>0.05).The similarity of sample fingerprint before and after sterilization was>0.9.In conclusion,instantaneous high temperature sterilization had no effect on the quality of Ilex kudingcha,which would provide experimental basis for the application of instantaneous high temperature sterilization technology in

关 键 词：苦丁茶冬青 瞬时高温灭菌 正交设计 灭菌率 抗氧化活性 多酚类化合物 

分 类 号：TQ460.61[化学工程—制药化工]