中国毛虾活性肽对免疫抑制小鼠免疫调节作用的影响  

Effects of Peptides from Acetes chinensis on Immunoregulation in Immunocompromised Mice

作　　者：杨志艳 惠婷婷 祝宝华 徐晨晨 李燕[1,2,3] 李晓晖 YANG Zhiyan;HUI Tingting;ZHU Baohua;XU Chenchen;LI Yan;LI Xiaohui(College of Food Science and Tecnology,Shanghai Ocean University,Shanghai 201306,China;Shanghai Aquatic Product and Processing and Storage Engineering Technology Research Center,Shanghai 201306,China;Laboratory for Risk Assessment of Quality and Safety of Storage and Preservation of Aquatic Products,Ministry of Agriculture,Shanghai 201306,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海水产品加工及贮藏工程技术研究中心,上海201306 [3]农业部水产品贮藏保鲜质量安全风险评估实验室(上海),上海201306

出　　处：《食品工业科技》2023年第9期380-386,共7页Science and Technology of Food Industry

基　　金：国家重点研发计划(低值水产品及副产物高值化利用与新产品创制,2019YFD0902000)。

摘　　要：本文以毛虾为原料,采用蛋白酶酶解获得毛虾活性肽,通过建立免疫抑制小鼠模型探究了毛虾活性肽对免疫抑制小鼠的免疫调节功能。小鼠随机分为六组:正常对照组、模型组、阳性对照组以及低、中、高剂量组,其中剂量组分别灌胃0.25 g·kg^(-1)BW、0.5 g·kg^(-1)BW、1.0 g·kg^(-1)BW毛虾活性肽。结果表明,剂量组免疫抑制小鼠体重明显增加(P<0.05),免疫器官指数中的胸腺指数显著提高(P<0.05),此外,毛虾活性肽还促进了小鼠血清中免疫球蛋白(IgA、IgG和IgM)以及细胞因子(IL-2、IL-6和TNF-α)水平的提升(P<0.05),外周血白细胞总数基本恢复至正常水平,NK细胞活性也显著增强(P<0.05)。综上,毛虾活性肽具有改善免疫抑制剂导致的免疫功能损伤,增强免疫抑制小鼠免疫调节的作用。In this paper,the active peptides were obtained from Acetes chinensis with protease,and then the immunoregulatory function and mechanism of them on the immunosuppressed mice were explored by establishing an immunosuppressive mouse model.Mice were randomly divided into six groups:Normal control group,model group,positive control group and low,medium and high dose groups,among which,the dose groups were given 0.25 g·kg^(−1)BW,0.5 g·kg^(−1)BW,1.0 g·kg^(−1)BW A.chinensis active peptide.The results showed that the body weight of the immunosuppressed mice in dose groups was significantly increased(P<0.05),and the thymus index of the immune organ indexes were remarkably improved(P<0.05)after being stimulated by the active peptides of Acetes chinensis.The serum levels of immunoglobulins(IgA,IgG,and IgM)and cytokines(IL-2,IL-6,and TNF-α)in mice were enhanced(P<0.05),and the total number of peripheral blood leukocytes was increased.In addition,NK cells activity was also significantly enhanced(P<0.05).To sum up,the active peptides of A.chinensis could improve the immune function damage caused by immunosuppression,and the immunoregulatory function of immunosuppressed mice.

关 键 词：中国毛虾 酶解 免疫抑制小鼠 免疫调节 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]