β-葡萄糖苷酶对甜菊糖苷的水解作用条件  

Hydrolysis Conditions of Stevioside by β-glucosidase

作　　者：童红梅 宋巧 张敏 郭敏 何丹 TONG Hongmei;SONG Qiao;ZHANG Min;GUO Min;HE Dan(Gansu Medical College,Pingliang 744000)

机构地区：[1]甘肃医学院,平凉744000

出　　处：《食品工业》2023年第3期181-184,共4页The Food Industry

基　　金：甘肃医学院青年基金(甜叶菊β-葡萄糖苷酶糖苷酶的分离纯化及酶学特性的研究(GYSF2021-02));甘肃医学院校级(β-葡萄糖苷酶对甜菊糖苷生物转化作用与调控机制研究GY-2022ZK006);平凉市科技局重点研发计划(陇东地区甜叶菊产业化试验引种技术研究PLKJJH2021003)。

摘　　要：探讨β-葡萄糖苷酶在甜菊糖苷生物转化中的作用。通过添加不同浓度β-葡萄糖苷酶水解乙醇超声提取的甜菊糖苷,探究最适酶浓度、温度与pH条件,用HPLC法测定甜菊糖苷、莱鲍迪苷A与斯替夫苷的含量,以确定β-葡萄糖苷酶水解作用效果。结果表明,在pH 7.0时添加600 U/g的β-葡萄糖苷酶,于乙醇溶液中超声(44.7 Hz、60℃、1 h)、水解(40℃、2 h)提取的甜菊糖苷,用HPLC测定甜叶菊茎秆中甜菊糖苷含量为0.167 mg/g,莱鲍迪苷A含量为0.125 mg/g,斯替夫苷含量为0.022 mg/g;叶片中甜菊糖苷含量为0.184 mg/g,莱鲍迪苷A含量为0.153 mg/g,斯替夫苷含量为0.035 mg/g,叶片甜菊糖苷含量高于茎秆;与未经β-葡萄糖苷酶处理对照相比,甜叶菊茎秆中甜菊糖苷含量提高28.7%,莱孢迪苷A含量提高23.7%,斯替夫苷含量提高15.8%;叶片中甜菊糖苷含量提高19.9%,莱孢迪苷A含量提高25.7%,斯替夫苷含量提高21.9%。β-葡萄糖苷酶可有效水解甜叶菊茎秆与叶片中的糖苷类物质,提高甜菊糖苷得率,茎秆中的甜菊糖苷也得以被提取利用。To investigate the role ofβ-glucosidase in the biotransformation of stevia,by adding different concentration ofβ-glycosidase enzymes hydrolysis ethanol ultrasonic extraction of stevia glycoside,the optimum enzyme concentration,temperature and pH condition was studied,and stevia sugar was determined by HPLC method,rebaudioside-A(RA)and stevioside(St)concentration,to determine theβ-glycosidase enzymes hydrolysis effect.The results showed that at pH7.0,600 U/gβ-glucosidase was added,and the stevioside extracted by ultrasonic ethanol was hydrolyzed at 40℃(60℃,44.7 Hz,2 h).The content of stevioside in stevia stem was 0.167 mg/g,and the content of reb A was 0.125 mg/g.The content of stevenside was 0.022 mg/g;The content of stevioside in leaves was 0.184 mg/g,the content of reboudiside A was 0.153 mg/g,the content of stevioside in leaves was 0.035 mg/g,and the content of stevioside in leaves was higher than that in stems.Compared with the control without β-glucosidase treatment,the content of stevioside in the stem of Stevia was increased by 28.7%,the content of lysine was increased by 23.7%,and the content of stevioside was increased by 15.8%.The content of stevioside in leaves was increased by 19.9%,the content of lysine was increased by 25.7%,and the content of stevioside was increased by 21.9%.Theβ-glucosidase could effectively hydrolyze the glycosides in the stem and leaves of Stevia,which could improve the yield of stevia,and the stevia in the stem could also be extracted and utilized.

关 键 词：Β-葡萄糖苷酶 甜菊糖苷 莱孢迪苷 斯替夫苷 水解作用 

分 类 号：TS201.2[轻工技术与工程—食品科学]