Anti-diabetic potential of apigenin,luteolin,and baicalein via partially activating PI3K/Akt/GLUT-4 signaling pathways in insulin-resistant HepG2 cells  

作　　者：Lingchao Miao Haolin Zhang Meng Sam Cheong Ruting Zhong Paula Garcia-Oliveira Miguel A.Prieto Ka-Wing Cheng Mingfu Wang Hui Cao Shaoping Nie Jesus Simal-Gandara Wai San Cheang Jianbo Xiao 

机构地区：[1]Institute of Chinese Medical Sciences,University of Macao,Macao 999078,China [2]Department of Analytical Chemistry and Food Science,Faculty of Food Science and Technology,University of Vigo-Ourense Campus,Ourense E-32004,Spain [3]Institute for Advanced Study,Shenzhen University,Shenzhen 508060,China [4]School of Biological Sciences,The University of Hong Kong,Hong Kong 999077,China [5]College of Food Science and Technology,Guangdong Ocean University,Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety,Guangdong Province Engineering Laboratory for Marine Biological Products,Guangdong Provincial Engineering Technology Research Center of Seafood,Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution,Zhanjiang 524088,China [6]State Key Laboratory of Food Science and Technology,China-Canada Joint Lab of Food Science and Technology(Nanchang),Nanchang University,Nanchang 330047,China [7]International Research Center for Food Nutrition and Safety,Jiangsu University,Zhenjiang 212013,China

出　　处：《Food Science and Human Wellness》2023年第6期1991-2000,共10页食品科学与人类健康（英文）

基　　金：supported by National Natural Science Foundation of China(32072212);Multi-Year Research Grant of University of Macao(MYRG2018-00169-ICMS);Science and Technology Development Fund of Macao(FDCT)(0098/2020/A);MICINN supporting the Ramón y Cajal grant for M.A.Prieto(RYC-201722891);Jianbo Xiao(RYC2020-030365-I);Xunta de Galicia supporting the Axudas Conecta Peme,the IN852A 2018/58 Neuro Food Project,the program EXCELENCIA-ED431F 2020/12;the pre-doctoral grants of P.García-Oliveira(ED481A-2019/295);to Ibero-American Program on Science and Technology(CYTED-AQUA-CIBUS,P317RT0003).

摘　　要：Dietary flavonoids are abundant in natural plants and possess multiple pharmacological and nutritional activities.In this study,apigenin,luteolin,and baicalein were chosen to evaluate their anti-diabetic effect in high-glucose and dexamethasone induced insulin-resistant(IR)HepG2 cells.All flavonoids improves the glucose consumption and glycogen synthesis abilities in IR-HepG2 cells via activating glucose transporter protein 4(GLUT4)and phosphor-glycogen synthase kinase(GSK-3β).These fl avonoids signifi cantly inhibited the production of reactive oxygen species(ROS)and advanced glycation end-products(AGEs),which were closely related to the suppression of the phosphorylation form of NF-κB and P65.The expression levels of insulin receptor substrate-1(IRS-1),insulin receptor substrate-2(IRS-2)and phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)pathway in IR-HepG2 cells were all partially activated by the fl avonoids,with variable effects.Furthermore,the intracellular metabolic conditions of the fl avonoids were also evaluated.

关 键 词：APIGENIN LUTEOLIN BAICALEIN Insulin-resistant HepG2 cells Signaling pathway Reactive oxygen species(ROS) Advanced glycation end-products(AGEs) Glycogen synthase kinase(GSK-3β) Glucose transporter protein 4(GLUT4) 

分 类 号：TS201.4[轻工技术与工程—食品科学]