不同养殖模式大黄鱼非标记定量差异蛋白组学分析  

Label-free Quantitative Differential Proteomic Analysis of Large Yellow Croaker Cultured under Different Aquaculture Modes

作　　者：翁丽萍[1] 张乐[1] 刘军波[1] 肖文斐[1] 汪前 邹礼根[1] WENG Liping;ZHANG Le;LIU Junbo;XIAO Wenfei;WANG Qian;ZOU Ligen(Hangzhou Academy of Agricultural Sciences,Hangzhou 310024,China)

机构地区：[1]杭州市农业科学研究院,浙江杭州310024

出　　处：《食品科学》2023年第8期137-142,共6页Food Science

基　　金：国家自然科学基金青年科学基金项目(31301463);浙江省水产品质量安全技术支撑团队项目(QS2019004);杭州市农科院科技创新与示范推广基金项目(2022HNCT-12)。

摘　　要：通过非标记定量蛋白质组学技术研究不同养殖模式大黄鱼的蛋白质差异。选取普通网箱养殖大黄鱼和深水网箱养殖大黄鱼,提取肌肉总蛋白,通过高效液相色谱-质谱联用技术进行鉴定、分析。质谱数据使用MaxQuant软件将峰信号转化为肽段/蛋白的表达矩阵数据,然后使用Perseus软件可视化展示数据。不同养殖模式大黄鱼蛋白质进行Student’s t检验,筛选出差异表达蛋白后并对其进行基因功能分类、代谢通路富集和蛋白质相互作用网络分析,以P<0.05为差异有统计学意义。通过数据库比对获得6077条多肽,分别对应于1232个蛋白,其中130个蛋白在大黄鱼间存在显著差异表达,76个上调蛋白,54个下调蛋白,其中上调倍数最高的属于TCP伴侣蛋白,下调倍数最高的属于α-1(I)链状胶原蛋白。生物信息学分析发现,不同养殖模式大黄鱼蛋白质的差异主要在于热休克蛋白、伴侣蛋白、球蛋白及胶原蛋白等,而不同养殖模式中环境温度可能是导致大黄鱼蛋白质显著差异表达的主要因素。Protein differences in large yellow croakers from different aquaculture patterns were investigated by label-free quantitative proteomics.Total muscle protein was extracted from large yellow croakers from common cage aquaculture and deep-water cage aquaculture,and identified and analyzed by high performance liquid chromatography-mass spectrometry(HPLC-MS).The raw data were processed using MaxQuant software to transform the signal peaks into matrix data for peptide/protein expression,followed by graphical visualization using the Perseus software.Differentially expressed proteins between the two aquaculture systems were selected by Student’s t test for gene functional classification,metabolic pathway enrichment and protein network interaction analysis,in which P<0.05 was considered as statistically significant difference.The results showed that 6077 peptides,corresponding to 1232 proteins,were obtained through database alignment.of these proteins,130 were significantly different between the two groups of large yellow croaker,with 76 being up-regulated proteins and 54 being down-regulated.The most up-regulated protein was TCP chaperone,and the most down-regulated protein wasα-1(I)chain collagen.Bioinformatics analysis demonstrated that the major differential proteins were heat shock protein,chaperones,globulin and collagen,and the environmental temperature may be the major factor causing significantly differential expression of large yellow croaker proteins.

关 键 词：大黄鱼 非标记定量 养殖模式 蛋白组学 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]