响应面优化藏羊皮胶原蛋白肽超声辅助提取工艺及其体内抗氧化活性分析  

Optimization of Ultrasonic Assisted Extraction of Tibetan Sheep Skin Collagen Peptide by Response Surface Methodology and Its Antioxidant Activity in Vivo

作　　者：牛金鸽 吴海玥[2] 马世科 闫忠心[2] 王学江 李婧 胡蓉[2] 祁全青 NIU Jinge;WU Haiyue;MA Shike;YAN Zhongxin;WANG Xuejiang;LI Jing;HU Rong;QI Quanqing(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China;Qinghai Academy of Animal Science and Veterinary Science,Qinghai University,Xining 810016,China;Qinghai Rural Industry Development Guidance Center,Xining 810000,China)

机构地区：[1]青海大学农牧学院,青海西宁810016 [2]青海大学畜牧兽医科学院,青海西宁810016 [3]青海省乡村产业发展指导中心,青海西宁810000

出　　处：《食品工业科技》2023年第11期163-170,共8页Science and Technology of Food Industry

基　　金：青海省昆仑英才高端创新创业人才项目(k992178);“藏羊产品加工标准化体系建设与示范”项目;青海省科技厅项目(2021-SF-C22);西宁市科技计划(2022-Y-11)。

摘　　要：为明确超声辅助提取对藏羊皮胶原蛋白肽含量及功能活性的影响。试验采用响应面法研究了料液比、超声功率和超声时间3个因素,确定了胶原蛋白肽最佳超声辅助提取工艺;并对藏羊皮胶原蛋白肽进行了抗氧化能力分析,模拟消化过程中的还原力和·OH清除能力研究。结果表明:超声辅助处理能明显提高胶原蛋白肽含量,最佳超声提取工艺为料液比1:18、超声功率220 W,超声时间27 min,藏羊皮胶原蛋白肽含量为30.21%±1.67%。超声波辅助提取后胶原肽的还原力和·OH清除率为0.49和83.2%,与超声前的胶原肽相比分别提高了4%和38.3%,经胃肠消化后还原力和·OH均有一定程度的下降,但仍然具有较好的抗氧化活性。超声辅助提取藏羊皮胶原蛋白肽具有提高胶原蛋白肽含量和增强体内抗氧化活性的优势,为功能性产品的开发提供了数据参考。In order to determine the effect of ultrasonic extraction on the content and functional activity of collagen peptide from Tibetan sheep skin,solid-liquid ratio,ultrasonic power and ultrasonic time were studied by response surface method to determine the best ultrasonic extraction process of collagen peptide.The antioxidant capacity,reduction power and·OH scavenging ability of collagen peptide from Tibetan sheep skin was analyzed.The results showed that ultrasound-assisted treatment had significantly affected the content of collagen peptide and antioxidant activity.The optimal ultrasonic extraction conditions were solid-liquid ratio 1:18,ultrasonic power 220 W and ultrasonic time 27 min.The collagen peptide content of Tibetan sheep skin was 30.21%±1.67%.The reduction power and·OH scavenging rate of collagen peptide after ultrasonic assisted extraction were 0.49 and 83.2%,which were increased by 4%and 38.3%compared with those before ultrasonic extraction,respectively.After gastrointestinal digestion,the reduction power and·OH decreased to a certain extent,but still had good antioxidant activity.Ultrasound-assisted extraction of collagen peptide from Tibetan sheep skin has the advantages of increasing the content of collagen peptide and enhancing antioxidant activity in vivo,which provides data reference for the development of functional products.

关 键 词：胶原肽 超声辅助提取 响应面法 抗氧化活性 

分 类 号：TS201[轻工技术与工程—食品科学]