云南木姜子提取物对蜡样芽孢杆菌抑菌作用的研究  

Study on the bacteriostatic effect of the extract of Yunnan Litsea cubeba on Bacillus cereus

作　　者：魏美娟 陈聪 牛刚 胡永金[1] 朱仁萍 朱仁俊[1] 薛桥丽 WEI Mei-Juan;CHEN Cong;NIU Gang;HU Yong-Jin;ZHU Ren-Ping;ZHU Ren-Jun;XUE Qiao-Li(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Zhaotong Food and Drug Inspection Institute,Zhaotong 657000,China;Editorial Office of Journal of Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学食品科学技术学院,昆明650201 [2]昭通市食品药品检验所,昭通657000 [3]云南农业大学学报编辑部,昆明650201

出　　处：《食品安全质量检测学报》2023年第9期43-48,共6页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(32060520)。

摘　　要：目的研究云南木姜子(Yunnan Litsea cubeba)的抑菌活性及抑菌稳定性。方法用乙醇对云南木姜子果实进行提取并分别用石油醚、氯仿、乙酸乙酯、正丁醇进行萃取。比较不同萃取相对蜡样芽孢杆菌的抑制作用,并通过热处理、酸碱处理、紫外照射、金属离子处理、糖和盐处理对其稳定性进行考察。结果云南木姜子提取物对蜡样芽孢杆菌具有良好的抑菌作用,其活性成分主要分布于乙酸乙酯相,其对蜡样芽孢杆菌的最小抑菌浓度为0.08 mg/mL;云南木姜子乙酸乙酯萃取相经紫外线照射、酸碱处理和不同温度处理仍表现出良好的稳定性,盐和糖处理条件使其对蜡样芽孢杆菌的抑菌活性增强,对于金属离子处理,Mg^(2+)使其对蜡样芽孢杆菌的抑菌活性提高,Fe^(3+)、Fe^(2+)、Al^(3+)和Ca^(2+)等使其抑菌活性降低。结论云南木姜子提取物具有良好的抑菌活性,温度、紫外和pH对其抑菌成分的稳定性有较小的影响,金属离子、糖和盐浓度对其抑菌活性影响较大。因此可考虑将云南木姜子作为一种天然植物源防腐剂,它在保鲜方面具有良好的开发和利用前景。Objective To study the antibacterial activity and stability of Yunnan Litsea cubeba.Methods The fruit of Litsea cubeba was extracted with ethanol and extracted with petroleum ether,chloroform,ethyl acetate and n-butanol respectively.The inhibitory effect of different extraction phases on Bacillus cereus was compared,and its stability was investigated by heat treatment,acid-base treatment,ultraviolet irradiation,metal ion treatment,sugar and salt treatment.Results The extract of Yunnan Litsea cubeba had a good antibacterial effect on Bacillus cereus.Its active components were mainly distributed in the ethyl acetate phase,and its minimum inhibitory concentration against Bacillus cereus was 0.08 mg/mL;the ethyl acetate extract of Litsea cubeba showed good stability after ultraviolet irradiation,acid-base treatment and different temperature treatment.The salt and sugar treatment conditions enhanced its antibacterial activity against Bacillus cereus.For metal ion treatment,Mg^(2+)increased its antibacterial activity against Bacillus cereus,while Fe^(3+),Fe^(2+),Al^(3+)and Ca^(2+)decreased its antibacterial activity.Conclusion The extract of Litsea cubeba has good antibacterial activity.Temperature,ultraviolet irradiation and pH have a small impact on the stability of antibacterial components,while metal ions,sugar and salt concentrations have a greater impact on their antibacterial activity.Therefore,Yunnan Litsea cubeba can be considered as a natural plant-derived preservative,which has good prospects for development and utilization in terms of preservation.

关 键 词：云南木姜子 蜡样芽孢杆菌 抑菌活性 稳定性 

分 类 号：TS201.3[轻工技术与工程—食品科学]