传统发酵肉源乳酸菌介导NPC1L1、ABCG5/G8基因表达对胆固醇代谢的影响  

Effect of Traditional Fermented Meat-derived Lactic Acid Bacteria Mediated NPC1L1 and ABCG5/G8 Gene Expression on Cholesterol Metabolism

作　　者：曹凯慧 张开屏[2] 马俊杰 程峰 杨雪倩 田建军[1] 靳烨[1] Cao Kaihui;Zhang Kaiping;Ma Junjie;Cheng Feng;Yang Xueqian;Tian Jianjun;Jin Ye(College of Food Science and Technology,Inner Mongolia Agricultural University,Hohhot 010018;Department of Cooking&Food Processing,Inner Mongolia Business and Trade Vocational College,Hohhot 010070)

机构地区：[1]内蒙古农业大学食品科学与工程学院,呼和浩特010018 [2]内蒙古商贸职业学院餐饮食品系,呼和浩特010070

出　　处：《中国食品学报》2023年第5期96-104,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金地区项目(31960514);内蒙古自治区科技计划项目(2019GG239);内蒙古自治区科技成果转化项目(2019CG066);内蒙古自然科学基金项目(2021MS03090)。

摘　　要：过高的胆固醇摄入可能会导致机体心脑血管出现健康问题。通过乳酸菌可以调控小肠中胆固醇代谢相关基因的表达,调节机体内胆固醇的吸收代谢,从而影响体内胆固醇含量。对15株传统发酵肉制品中分离筛选的乳酸菌进行体外降胆固醇能力和耐酸耐胆盐能力筛选,将筛选到的乳酸菌用于动物实验。结果表明,15株乳酸菌中有4株乳酸菌(CM6、CM9、CM28、CM42)具有良好的体外降胆固醇能力,平均降解率为52.92%。对它们进行耐酸和耐胆盐试验,在pH 2和0.3%胆盐添加量的条件下菌株CM6活菌数达到106 CFU/mL,选择CM6进行后续动物实验。乳酸菌灌胃小鼠30 d后,乳酸菌组小鼠血清中总胆固醇含量和甘油三酯含量乳显著低于对照组(P<0.05);乳酸菌组小鼠小肠中NPC1L1基因相对表达量显著低于对照组(P<0.05),ABCG5/G8基因表达量显著高于对照组(P<0.05)结论:乳酸菌CM6具有调控胆固醇代谢相关基因表达的能力,从而降低体内胆固醇含量。Excessive cholesterol intake may lead to cardiovascular and cerebrovascular health problems.Lactic acid bacteria can regulate the absorption and metabolism of cholesterol in the body by regulating the expression of cholesterol metabolism related genes in the small intestine,thus affecting the cholesterol content in the body.In this study,15 lactic acid bacteria isolated from traditional fermented meat products were screened for cholesterol-lowering ability,acid and bile salt tolerance in vitro,and the screened lactic acid bacteria were used in animal experiments.The results showed that 4 of the 15 lactic acid bacteria(CM6,CM9,CM28,CM42)had good cholesterol-lowering ability in vitro,and the average degradation rate was 52.92%.Acid and bile salt tolerance tests were carried out on these four lactic acid bacteria strains,among which strain CM6 could reach 106 CFU/mL at pH 2 and 0.3%bile salt addition.Finally,CM6 was selected for subsequent animal experiments.After 30 days of intragastric administration of lactic acid bacteria,the contents of total cholesterol and triglyceride in serum of mice in the lactic acid bacteria group were significantly lower than those in the control group(P<0.05).The relative expression of NPC1L1 gene in the small intestine of the lactic acid bacteria group was significantly lower than that of the control group(P<0.05),and the expression of ABCG5/G8 gene was significantly higher than that of the control group(P<0.05).The results showed that lactic acid bacteria CM6 had the ability to regulate the expression of cholesterol metabolism-related genes,thereby reducing the cholesterol content in the body.

关 键 词：乳酸菌 胆固醇 NPC1L1 ABCG5/G8 基因相对表达量 

分 类 号：TS201.3[轻工技术与工程—食品科学]