UPLC-MS/MS法同时测定甘草中6种人工合成甜味剂  

Simultaneous determination of six artificial sweeteners in Glycyrrhizae Radix by UPLC-MS/MS

作　　者：朱泽兵 郭娅 张德伟 ZHU Zebing;GUO Ya;ZHANG Dewei(Chongqing Key Laboratory of Development and Utilization of Dao-di Herbs in the Three Gorges Reservoir Area,Chongqing Wanzhou Food and Drug Inspection Institute,Wanzhou,Chongqing,404100 P.R.China)

机构地区：[1]重庆市万州食品药品检验所三峡库区道地药材开发利用重庆市重点实验室,重庆万州404000

出　　处：《华西药学杂志》2023年第3期310-313,共4页West China Journal of Pharmaceutical Sciences

基　　金：重庆市药品监督管理局和重庆市科学技术局(渝药监[2021]30号)。

摘　　要：目的采用超高效液相色谱-四级杆/静电场轨道阱高分辨质谱法同时测定甘草中甜蜜素、糖精钠、安赛蜜、阿斯巴甜、阿力甜、纽甜等6种人工合成甜味剂。方法HPLC采用Hypersil GOLD C18色谱柱(100 mm×2.1 mm,3μm),流动相为乙腈-0.1%甲酸5 mmol·L^(-1)甲酸铵溶液,梯度洗脱,体积流量0.30 mL·min^(-1),进样量5μL;质谱采用电喷雾离子源、负离子模式采集,通过多反应监测同时对甘草中6种人工合成甜味剂进行定量分析。结果甘草中甜蜜素、糖精钠、安赛蜜、阿斯巴甜、阿力甜、纽甜等的线性范围分别为10.899~435.96、11.18~447.2、10.0~400.0、11.352~454.08、10.88~435.2、10.172~406.88 ng·mL^(-1)(r分别为0.9986、0.9991、0.9975、0.9992、0.9971、0.9989),平均回收率为102.54%~113.66%,RSD为1.75%~3.11%(n=6)。结论所用方法快速、高效,灵敏度高、专属性好,可应用于甘草中人工合成甜味剂的鉴别。OBJECTIVE To simultaneously determine cypress,saccharin sodium,acesulfamine,aspartame,alidame and neotame in Glycyrrhizae Radix by ULTRA performance liquid chromatography-quadrupole/electrostatic field Orbitrap high resolution mass spectrometry.METHODS HPLC was performed on Hypersil GOLD C18 column(100 mm×2.1 mm,3μm)with mobile phase consisting of acetonitrile-0.1%formic acid 5 mmol·L^(-1) ammonium formate solution,gradient elution,volume flow of 0.30 mL·min^(-1),sample volume of 5μL.Six artificial sweeteners in G.Radix were quantitatively analyzed simultaneously by multiple reaction monitoring.RESULTS The linear ranges of cycetin,saccharin sodium,acesulmine,aspartame,alidame and neutame were 10.899-435.96,11.18-447.2,10.0-400.0,11.352-454.08,10.88-435.2,10.172-406.88 ng·mL^(-1)(r=0.9986,0.9991,0.9975,0.9992,0.9971 and 0.9989),respectively.The average recoveries were 102.54%-113.66%,with RSD 1.75%-3.11%(n=6).CONCLUSION The established method is rapid,efficient,sensitive and specific,and can be applied to the identification of artificial sweeteners in G.Radix.

关 键 词：超高效液相色谱-质谱联用法 甘草 人工合成甜味剂 甜蜜素 糖精钠 安赛蜜 阿斯巴甜 阿力甜 纽甜 

分 类 号：R917[医药卫生—药物分析学]