高效液相色谱法同时测定窖泥和养窖液中四种有机酸  

Simultaneous Detection of Four Organic Acids in Pit Mud and Functional Bacteria Liquid by HPLC

作　　者：梅婕 李玉岩 程凡 李赛 张显[3] 张温清 MEI Jie;LI Yuyan;CHENG Fan;LI Sai;ZHANG Xian;ZHANG Wenqing(Technology Research Center of Xuanjiu Group Co.Ltd.,Xuancheng,Anhui 242000;Jiangnan Institute of Small Pit Brewing Technology,Xuancheng,Anhui 242000;School of Biotechnology,Jiangnan University,Wuxi,Jiangsu 214122,China)

机构地区：[1]安徽宣酒集团股份有限公司科研中心,安徽宣城242000 [2]江南小窖酿造工艺研究所,安徽宣城242000 [3]江南大学生物工程学院,江苏无锡214122

出　　处：《酿酒科技》2023年第7期102-106,共5页Liquor-Making Science & Technology

摘　　要：建立了高效液相色谱快速测定窖泥和养窖液中4种有机酸的方法。以甲醇和磷酸水溶液为流动相,通过梯度洗脱,在C18色谱柱中进行分离。该方法线性关系良好,R2大于0.9990,样品的回收率为90.26%~103.95%,相对标准偏差小于5.99%,适用于日常分析。In this study,the content of lactic acid,acetic acid,butyric acid and hexanoic acid in pit mud and functional bacteria liquid was detected simultaneously by HPLC.The samples were separated by C18 column with gradient elution.Carbinol and H3PO4 solution were used as the mobile phase.This method possessed good linear relationship(R2>0.9990).The recovery rates were between 90.26%and 103.95%,and the relative standard deviations(RSD)were lower than 5.99%.The method is suitable for daily analysis.

关 键 词：高效液相色谱 乳酸 乙酸 丁酸 己酸 窖泥 养窖液 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS261.7[轻工技术与工程—食品科学与工程]