榛蘑液体培养基优化及其多糖抗氧化性评价  

Optimization of liquid culture medium of Armillaria mellea and evaluation of its polysaccharide antioxidant activity

作　　者：贾健辉 刘颖 贾婷婷 辛思滢 张彦丽[1] JIA Jian-hui;LIU Ying;JIA Ting-ting;XIN Si-ying;ZHANG Yan-li(College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang 157011,Heilongjiang,China)

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157011

出　　处：《粮食与油脂》2023年第7期109-113,共5页Cereals & Oils

基　　金：黑龙江省教育厅科研重点项目(1355ZD008)。

摘　　要：通过单因素试验来筛选碳源、氮源和无机盐的种类,通过响应面试验优化榛蘑液体菌种培养基配方,并研究菌丝体多糖体外抗氧化活性。结果表明:当以蔗糖作为碳源、蛋白胨作为氮源和磷酸二氢钾作为无机盐时,菌丝体多糖含量和生物量最高。榛蘑液体菌种培养基最优配方为蔗糖添加量33.0 g/L、蛋白胨添加量21.6 g/L、磷酸二氢钾添加量1.7 g/L,在此条件下菌丝体多糖含量达到8.91 g/100 g。热水浸提法得到的菌丝体多糖具有清除DPPH·、ABTS^(+)·、·OH的能力,说明榛蘑菌丝体多糖具有一定的抗氧化活性。The types of carbon source,nitrogen source and inorganic salt were screened by single factor test.The formula of liquid culture medium of Armillaria mellea was optimized by response surface test,and the in vitro antioxidant activity of mycelium polysaccharide was studied.The results showed that the polysaccharide content and biomass of mycelium were the highest when sucrose was used as carbon source,peptone as nitrogen source and potassium dihydrogen phosphate as inorganic salt.The optimal formula of liquid culture medium of Armillaria melea was sucrose supplemental level of 33.0 g/L,peptone supplemental level of 21.6 g/L,potassium dihydrogen phosphate supplemental level of 1.7 g/L,under these conditions,mycelium polysaccharide content reached 8.91 g/100 g.The mycelium polysaccharide obtained by hot water extraction had the ability to clear DPPH·,ABTS^(+).and·OH,which indicated that the mycelium polysaccharide had certain antioxidant activity.

关 键 词：榛蘑 菌丝体 培养基 多糖 抗氧化性 

分 类 号：TS201.2[轻工技术与工程—食品科学]