紫苋籽粒乙醇提取工艺及其抗氧化性研究  

Ethanol Extraction Process and Antioxidant Study on Purple Amaranth Grain

作　　者：王宇婷 金铁岩[1] 王云峰 王龙宇 张智勇 熊思睿 WANG Yuting;JIN Tieyan;WANG Yunfeng;WANG Longyu;ZHANG Zhiyong;XIONG Sirui(College of Agriculture,Yanbian University,Yanji 133002)

机构地区：[1]延边大学农学院,延吉133002

出　　处：《食品工业》2023年第6期68-72,共5页The Food Industry

摘　　要：为探究紫苋籽粒乙醇提取物(AEE)的最优工艺和抗氧化活性,以紫苋籽粒粉作为原料,试验通过控制提取时间、提取剂浓度以及料液比进行试验,并以单因素试验为依据进行正交试验,分析得出最优的提取条件,通过对1,1-二苯基-2-三硝基苯肼(DPPH)、ABTS自由基的清除能力和铁离子还原能力来评价乙醇提取物中多酚抗氧化能力。结果表明:提取多酚的最适条件是体积分数80%乙醇、1∶33 (g/mL)的料液比及提取时间12 h,此时紫苋籽粒中多酚平均提取率为33.64%;多酚对DPPH的清除率能力为75.43%,ABTS的清除率能力为61.02%,铁离子具备一定的还原能力,由此说明紫苋籽粒中多酚存在一定的抗氧化能力,且其质量浓度与抗氧化性具有量效关系。In order to explore the optimal process and antioxidant activity of the ethanol extract(AEE) from purple amaranth grain,using purple amaranth grain powder as raw material,the experiment was conducted by controlling the extraction time,extraction concentration and solid-liquid ratio,orthogonal test was conducted on the basis of single factor experiment,and the optimal extraction conditions were obtained.The antioxidant capacity of polyphenols in ethanol extract was evaluated by the scavenging ability of 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH·) and ABTS free radicals and the reduction ability of iron ion.The results showed that the optimal extraction conditions were 80% ethanol concentration,1∶33(g/mL) solid-liquid ratio and extraction time of 12 h,and the average extraction yield of polyphenols was 33.64%.The scavenging capacity of polyphenols to DPPH· was 75.43%,and that of ABTS was 61.02%.Iron ions had a certain reducing capacity,which indicated that polyphenols in Purslane grains had a certain antioxidant capacity,and its mass concentration had a dot-effect relationship with the antioxidant capacity.

关 键 词：紫苋籽粒 多酚 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]