食品中赭曲霉毒素A检测方法研究进展  

Advances in the detection methods of ochratoxin A in foods

作　　者：蒋彩云[1,3] 胡海祥 赵津津 李琰 JIANG Caiyun;HU Haixiang;ZHAO Jinjin;LI Yan(School of Health,Jiangsu Vocational Institute of Commerce,Nanjing,Jiangsu 211168,China;Alex Stewart(Agriculture)China LTD,Suzhou,Jiangsu 215300,China;Jiangsu Engineering and Research Center of Food Safety,Jiangsu Vocational Institute of Commerce,Nanjing,Jiangsu 211168,China)

机构地区：[1]江苏经贸职业技术学院健康学院,江苏南京211168 [2]江苏安舜技术服务有限公司,江苏苏州215300 [3]江苏经贸职业技术学院江苏省食品安全工程技术研发中心,江苏南京211168

出　　处：《贵州师范大学学报（自然科学版）》2023年第4期58-63,共6页Journal of Guizhou Normal University：Natural Sciences

基　　金：江苏省高校优秀科技创新团队(2021年);江苏省高等学校自然科学研究面上项目(21KJB430005);江苏省333高层次人才培养工程;江苏经贸职业技术学院领军人才项目。

摘　　要：赭曲霉毒素是一种丝状真菌毒素,有A、B、C、D 4种化合物,受其污染的食品会对人体产生危害,尤其是赭曲霉毒素A的危害最为突出。目前,对食品中赭曲霉毒素A的检测方法主要有薄层色谱法、高效液相色谱法、液相色谱-串联质谱法、酶联免疫法、电化学传感器法以及荧光分析法,笔者通过梳理、分析上述检测方法的优缺点,提出:薄层色谱法、酶联免疫吸附等传统方法可在灵敏度和准确性等方面提升;高效液相色谱、液相色谱-串联质谱法等相对比较成熟的方法可在成本、选择性上进一步优化;电化学传感器、荧光传感器等这些具有绿色性和创新性的检测方法,如在电极材料制备技术、核酸适配体筛选及修饰技术上再提高,其应用前景必将更加广泛。Ochratoxin is a filamentous mycotoxin with four compounds,A,B,C and D.The food contaminated by ochratoxin will do harm to human body,and the ochratoxin A is the particularly harmful.At present,the detection methods of ochratoxin A in food mainly include thin-layer chromatography,high-performance liquid chromatography,liquid chromatography-mass spectrometry,enzyme-linked immunosorbent assay,electrochemical sensor method and fluorescence analysis.By analyzing the advantages and disadvantages of the above detection methods,the author puts forward that traditional methods,such as thin-layer chromatography and enzyme-linked immunosorbent assay,can be improved in sensitivity and accuracy;Relatively sophisticated methods,such as liquid chromatography and liquid chromatography-mass spectrometry,can be further optimized in terms of cost and selectivity;Electrochemical sensors,fluorescent sensors and other green and innovative detection methods will have a wide application prospect if they are further improved in electrode material preparation technology,nucleic acid aptamer screening and modification technology.

关 键 词：真菌毒素 赭曲霉毒素A 检测方法 食品 

分 类 号：TS207.4[轻工技术与工程—食品科学] R37[轻工技术与工程—食品科学与工程]