金樱子总多酚提取工艺优化及抗氧化活性研究  

Optimization of extraction process and antioxidant activity of total polyphenols from Rosa laevigata

作　　者：王慧竹[1] 李新胜 王亮 陈帅[1] WANG Huizhu;LI Xinsheng;WANG Liang;CHEN Shuai(School of Chemistry and Pharmaceutical Engineering,Jilin Institute of Chemical Technology,Jilin 132022,China)

机构地区：[1]吉林化工学院化学与制药工程学院,吉林吉林132022

出　　处：《中国酿造》2023年第7期190-195,共6页China Brewing

基　　金：吉林省教育厅科学技术项目(JJKH20200256KJ);吉林化工学院博士启动基金项目(吉化院博基合字2019第[002]号)。

摘　　要：该研究采用超声提取法提取金樱子总多酚,以总多酚得率为评价指标,通过Plackett-Burman(PB)试验设计、单因素试验及Box-Behnken(BB)响应面法优化金樱子总多酚的提取工艺,并对其抗氧化活性进行评价。结果表明,金樱子总多酚最佳超声提取工艺为:液料比21∶1(mL∶g),乙醇体积分数61%,超声时间39 min,提取温度48℃。在此优化条件下,总多酚得率为3.84%,比优化前(2.74%)提高40.15%。金樱子总多酚具有较强的抗氧化活性,金樱子总多酚溶液质量浓度为1.2 mg/mL时,金樱子多酚对1,1-二苯基-2-三硝基苯肼(DPPH)及2,2'-联氨-双(3-乙基苯并噻唑啉-6-磺酸)二胺盐(ABTS+)自由基的半抑制浓度(IC50)值分别为0.502 mg/mL、0.425 mg/mL。In this study,total polyphenols were extracted from Rosa laevigata by ultrasonic extraction.Using total polyphenol yield as evaluation index,the extraction process of total polyphenols from R.laevigata was optimized by Plackett-Burman(PB)experimental design,single factor experiments combined with Box-Behnken(BB)response surface method,and its antioxidant activity was evaluated.The results showed that the optimum ultrasonic extraction process of total polyphenols from R.laevigata was as follows:liquid and material ratio 21∶1(ml∶g),ethanol volume fraction 61%,ultrasonic time 39 min,extraction temperature 48℃.Under these optimal conditions,the yield of total polyphenols was 3.84%,which was 40.15%higher than that before optimization(2.74%).The total polyphenols had strong antioxidant activity,when the concentration of polyphenol solution was 1.2 mg/ml,the half inhibition concentration(IC50)of 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diamine salt(ABTS+)free radical were 0.502 mg/ml and 0.425 mg/ml,respectively.

关 键 词：金樱子 总多酚 超声提取 Plackett-Burman试验设计 Box-Behnken响应面试验 抗氧化活性 

分 类 号：TS202[轻工技术与工程—食品科学]