微酸性电解水对采后西兰花叶绿素降解的影响  

Effect of Slightly Acidic Electrolyzed Water on Chlorophyll Degradation in Postharvest Broccoli

作　　者：韩颖 孙莹[1] 郭峰 王馨渝[1] 赵安琪 吴朝霞 李鹏霞[1,3] 李国锋[1,3] 胡花丽 HAN Ying;SUN Ying;GUO Feng;WANG Xinyu;ZHAO Anqi;WU Zhaoxia;LI Pengxia;LI Guofeng;HU Huali(Institute of Agricultural Facilities and Equipment,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;College of Food Science,Shenyang Agricultural University,Shenyang 110866,China;Key Laboratory of Cold Chain Logistics Technology for Agro-product,Ministry of Agriculture and Rural Affairs,Nanjing 210014,China)

机构地区：[1]江苏省农业科学院农业设施与装备研究所,江苏南京210014 [2]沈阳农业大学食品学院,辽宁沈阳110866 [3]农业农村部农产品冷链物流技术重点实验室,江苏南京210014

出　　处：《食品科学》2023年第13期139-149,共11页Food Science

基　　金：江苏省农业科技自主创新资金项目(CX(20)1008);江苏省重点研发计划(现代农业)-面上项目(BE2022368)。

摘　　要：为了探究微酸性电解水(slightly acidic electrolyzed water,SAEW)对采后西兰花叶绿素降解的影响,本研究以采后西兰花为材料,分析50 mg/L SAEW处理采后西兰花色差、总叶绿素含量、叶绿素衍生物含量、叶绿素降解酶活性和叶绿素代谢关键基因表达的变化规律。结果表明:与对照组相比,SAEW处理可有效减缓采后西兰花中总叶绿素的降解,维持其叶绿素相关衍生物叶绿素a、叶绿素b、脱植基叶绿素a、脱植基叶绿素b、脱镁叶绿素a和脱镁叶绿酸a的含量;抑制采后西兰花叶绿素酶、脱镁螯合酶、脱镁叶绿素酶和脱镁叶绿素a加氧酶活力的升高;同时显著抑制叶绿素b还原酶编码基因、叶绿素酶1编码基因、叶绿素酶2编码基因、滞绿蛋白编码基因、脱镁叶绿素酶编码基因、脱镁叶绿酸a氧化酶编码基因、红色叶绿素降解产物还原酶编码基因和衰老特异性半胱氨酸蛋白酶编码基因的表达,实现护色保鲜。综上,SAEW可作为一种延缓采后西兰花叶绿素降解、抑制其黄化衰老的有效处理方法。To explore the effect of slightly acidic electrolyzed water(SAEW)on chlorophyll degradation in postharvest broccoli,the pattern of changes in the color,total chlorophyll content,chlorophyll derivative content,chlorophyll degrading enzyme activities,and key chlorophyll metabolism-related gene expression of postharvest broccoli after treatment with 50 mg/L SAEW was analyzed.The results showed that SAEW treatment could effectively slow down the degradation of total chlorophyll,maintain the contents of chlorophyll derivatives chlorophyll a,chlorophyll b,chlorophyllide a,chlorophyllide b,pheophorbide a,and pheophytin a,and delay the increase in the activities of chlorophyll metabolizing enzymes,Mg-dechelatase,pheophytinase,and pheophorbide an oxygenase in postharvest broccoli.Meanwhile,it significantly inhibited the expression of the genes encoding chlorophyll b reductase,chlorophyllase 1,chlorophyllase 2,chlorosis protein,pheophytinase,pheophorbide an oxygenase,red chlorophyll catabolite reductase,and aging specific cysteine protease,thereby allowing color protection and freshness preservation.In conclusion,SAEW can be used as an effective method to delay postharvest chlorophyll degradation and inhibit yellowing and senescence in broccoli.

关 键 词：微酸性电解水 采后西兰花 叶绿素降解 黄化 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]