酪蛋白胶束与多元活性分子的相互作用及其复合物特性  

Interactions between Casein Micelles and Various Active Molecules and Properties of Their Complexes

作　　者：郑杰 杨敏[1,2] 甄晨波 秦娟娟 ZHENG Jie;YANG Min;ZHEN Chenbo;QIN Juanjuan(College of Science,Gansu Agricultural University,Lanzhou 730070,China;Institute of Agricultural Resources Chemistry and Application,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学理学院,甘肃兰州730070 [2]甘肃农业大学农业资源化学与应用研究所,甘肃兰州730070

出　　处：《食品科学》2023年第14期8-19,共12页Food Science

基　　金：国家自然科学基金地区科学基金项目(32060549);甘肃省自然科学基金项目(22JR5RA861);甘肃农业大学伏羲杰出人才培育计划项目(Gaufx-02J02)。

摘　　要：以酪蛋白胶束(casein micelles,MC)为基质,以单一原花青素(proanthocyanidin,PC)、儿茶素(catechin,Cat)、叶绿素铜钠(chlorophyllin sodium copper salt,Chl)或其二元组合为配体,通过热处理或热处理联合超声处理制备二元、三元复合物,系统分析了单一PC、Cat、Chl或其二元组合物与MC的相互作用,解析MC对多元分子的同时结合能力,表征复合物结构、微观形貌及热稳定性,探究三元复合物的抗氧化性以及体外模拟消化特性。结果表明,单一PC、Cat、Chl通过疏水相互作用与MC形成二元复合物,其中MC对Chl表现出较高的亲和力。热处理条件下,当MC以疏水作用结合一个分子后,另一个分子则以范德华力、氢键结合于MC上,且298 K时第1个分子的结合提升了第2个分子与MC的结合常数和结合位点数。PC、Cat、Chl单一分子或其二元组合物的结合对MC空间结构及微观形貌无显著影响。热处理条件下,活性分子的结合增加了MC的热稳定性。就抗氧化性而言,热处理改善了三元复合物的2,2-联氮双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基清除力,而热处理联合超声处理改善了1,1-二苯基-2-三硝基苯肼自由基清除活性。此外,多元活性分子的同时负载改善了MC在模拟肠液中的消化性。本研究结果可为MC在多元活性分子负载中的应用提供一定参考。In this study,binary and ternary complexes were prepared by heat treatment or combined heat and ultrasonic treatment using casein micelles(MC)as matrix and proanthocyanidin(PC),catechin(Cat)and chlorophyllin sodium copper salt(Chl)or their binary combinations as ligands.The interactions between PC,Cat,Chl or their binary combinations and MC were analyzed,and the binding capacity of MC to the various active molecules was evaluated.In addition,the structure,microscopic morphology and thermal stability of the complexes were characterized,and the antioxidant properties and in vitro digestibility of the ternary complexes were investigated as well.The results showed that PC,Cat and Chl formed binary complexes with MC through hydrophobic interactions,among which MC showed the highest affinity for Chl.Under heat treatment conditions,MC bound one molecule through hydrophobic interaction and another one by van der Waals forces and hydrogen bonds.At 298 K,the binding of the first molecule improved the binding constant and the number of binding sites of the second one.The binding of PC,Cat,Chl or their binary complexes had no significant effect on the spatial structure or microscopic morphology of MC.The thermal stability of MC was enhanced after the binding of bioactive molecules under heat treatment conditions.In terms of antioxidant properties,heat treatment promoted the 2,2’-azino bis-(3-ethylbenzthiazoline-6-sulfonicacid)(ABTS)radical cation scavenging activity of the ternary complexes,while combined heat and ultrasonic treatment promoted their 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging activity.In addition,the loading of active molecules improved the digestibility of MC in simulated intestinal fluid.This study could provide a reference for the application of MC in the loading of bioactive molecules.

关 键 词：原花青素 儿茶素 叶绿素铜钠 酪蛋白胶束 相互作用 抗氧化性 消化性 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]