基于比较基因组学解析植物乳植杆菌ST的功能基因组  

Comparative Genomic Analysis of Functional Genomics of Lactiplantibacillus plantarum ST

作　　者：杨淑娟 周金萍 李海燕 曹振辉[3] 孙志宏[1] 林秋叶[2] YANG Shujuan;ZHOU Jinping;LI Haiyan;CAO Zhenhui;SUN Zhihong;LIN Qiuye(Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education,Key Laboratory of Dairy Products Processing,Ministry of Agriculture and Rural Affairs,Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering,Collaborative Innovative Center of Ministry of Education for Lactic Acid Bacteria and Fermented Dairy Products,Inner Mongolia Agricultural University,Hohhot 010018,China;College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Faculty of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]内蒙古农业大学乳品生物技术与工程教育部重点实验室,农业农村部奶制品加工重点实验室,内蒙古自治区乳品生物技术与工程重点实验室,乳酸菌与发酵乳制品省部共建协同创新中心,内蒙古呼和浩特010018 [2]云南农业大学食品科学技术学院,云南昆明650201 [3]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《食品科学》2023年第14期86-93,共8页Food Science

基　　金：国家自然科学基金地区科学基金项目(31760448);云南省孙志宏专家工作站(202105AF150055);“兴滇英才支持计划”青年人才项目(YNWR-QNBJ-2018-137)。

摘　　要：解析植物乳植杆菌(Lactiplantibacillus plantarum)ST的功能基因组特征,为其开发利用奠定基础。对L.plantarum ST与1株模式菌株ATCC 14197T基因组序列及NCBI中已公开的152株L.plantarum全基因组序列进行比较基因组学分析。基于154株L.plantarum的1262个核心基因构建了系统发育树,发现ST与肠道分离株BCC9546遗传距离最近。乳、肉制品分离株分别集中在第II分支的上半部分和下半部分,因来源不同而存在差异,且存在一定的聚集性。同时果蝇分离株具有明显聚集趋势。功能注释分析发现,ST基因组中包含磷酸转移酶系统糖类转运系统相关基因,还注释到了免疫调控通路相关基因,同时有较多水解或重排糖苷键的基因。ST不存在毒力因子和耐药基因。与其他L.plantarum相比,ST携带特有的与能量转运功能有关的功能基因ecfT。此外,ST具有与群体感应信号分子AI-2、黏附分子和谷胱甘肽合成相关的功能基因。API 50 CHL碳水化合物代谢结果显示,L.plantarum ST能利用丰富的碳源,可代谢包括单糖类、糖苷和二糖类及多糖类等中29种碳源。本研究从基因组层面分析L.plantarum ST功能基因组特征,同时结合糖发酵表型实验结果,认为L.plantarum ST是1株安全且具有潜在益生特性的益生菌,为其开发利用提供遗传学基础。In this study,the functional genomic characteristics of Lactiplantibilus plantarum ST were analyzed.Comparative genomic analysis was performed on the whole genome sequences of L.plantarum ST and the type strain L.plantarum ATCC 14197T as well as those of 152 L.plantarum strains published in the NCBI GenBank.A phylogenetic tree was constructed based on 1262 core genes of these 154 L.plantarum strains,and the genetic distance between ST and the intestinal isolate BCC9546 was found to be the closest.The isolates originated from milk and meat products were concentrated in the upper part and the lower part of the second branch,respectively,and they were different due to different sources and could be aggregated.Meanwhile,Drosophila isolates showed a significant aggregation trend.Functional annotation analysis suggested that the ST genome contained genes related to the phosphotransferase system(PTS)as a major carbohydrate transport system.Genes related to the immune regulatory pathway were also annotated.There were many genes related to the hydrolysis or rearrangement of glycosidic bonds,but no virulence factors or antibiotics resistance genes found in ST.Compared with the other L.plantarum isolates,ST carried the unique functional gene ecfT related to energy transport function.In addition,ST had functional genes involved in the synthesis of the quorum-sensing signaling molecule AI-2,glutathione,and adhesion molecules.The results of API 50 CHL carbohydrate metabolism showed that L.plantarum ST could utilize a wide range of carbon sources and metabolize 29 carbon sources including monosaccharides,glycosides,disaccharides and polysaccharides.This study provides a genetic basis for the development and utilization of L.plantarum ST as a safe probiotic strain.

关 键 词：植物乳植杆菌ST 比较基因组学 功能基因 

分 类 号：TS201.3[轻工技术与工程—食品科学]