乳酸菌发酵天麻口服液工艺优化  

Optimization of Fermentation Processing of Gastrodia elata Oral Liquid with Lactic Acid Bacteria

作　　者：陈来凤 倪琳钰 邓成林 罗亚楠 李海燕 丰贵江 林秋叶[1] CHEN Laifeng;NI Linyu;DENG Chenglin;LUO Yanan;LI Haiyan;FENG Guijiang;LIN Qiuye(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学食品科学技术学院,云南昆明650201

出　　处：《食品工业科技》2023年第15期193-202,共10页Science and Technology of Food Industry

基　　金：“兴滇英才支持计划”青年人才项目(YNWR-QNBJ-2018-137)。

摘　　要：本研究以天麻为研究对象,优化复合酶法处理天麻工艺,利用课题组自主分离的植物乳植杆菌STDA6作为发酵菌剂制备发酵天麻口服液,采用正交试验优化乳酸菌发酵天麻口服液的制备工艺。结果表明,将果胶酶、纤维素酶、单宁酶和α-淀粉酶等比例混合后对天麻进行酶解处理,复合酶酶解最佳工艺条件为:料液比(g:m L)1:11,加酶量0.4%,p H4.0,酶解温度50℃,酶解时间180 min;乳酸菌发酵天麻口服液的最佳工艺条件为:接种量1×10^(5)CFU/m L,发酵时间12 h,发酵温度37℃,蔗糖添加量10%,采用上述工艺制备的乳酸菌发酵天麻口服液乳酸菌活菌数为2×10^(8)CFU/m L,p H为3.5,菌落总数<10 CFU/m L,大肠菌群、霉菌和酵母菌、金黄色葡萄球菌、沙门氏菌均未检出,符合GB/T 31326-2014《植物饮料》的相关要求。In this study,Gastrodia elata was treated with compound enzyme,and Lactiplantibacillus plantarum STDA6 was used as fermentation agent to prepare fermented G.elata oral liquid.Orthogonal experiment was used to optimize the preparation process of lactic acid bacteria fermented G.elata oral liquid.The results showed that the optimal process conditions for enzymatic hydrolysis of G.elata were carried out after mixing pectinase,cellulase,tannins andα-amylase,and the optimal process conditions for enzymatic hydrolysis of complex enzymatic hydrolysis were:the ratio of material to liquid(g:mL)was 1:11,the amount of enzyme was 0.4%,the pH value was 4.0,the enzymatic hydrolysis temperature was 50℃,the enzymatic hydrolysis time was 180 min.The optimum technological conditions of lactic acid bacteria fermenting G.elata oral liquid were as follows:inoculation amount with 1×10^(5)CFU/mL,fermentation time for 12 h,fermentation temperature at 37℃,sucrose addition with 10%.The average number of L.plantarum STDA6 in G.elata oral liquid fermented by lactic acid bacteria was 2×10^(8)CFU/mL,pH value was 3.5,the total number of bacteria was less than 10 CFU/mL and coliform,mold,yeast,Staphylococcus aureus,Salmonella were not been detected,which complied with the relevant requirements of GB/T 31326-2014‘Botanical Beverage’.

关 键 词：天麻 酶解 乳酸菌 植物乳植杆菌 

分 类 号：TS275.4[轻工技术与工程—农产品加工及贮藏工程]