南美白对虾副产物酶解工艺优化及其蛋白肽活性评价  

Optimization of enzymatic digestion process of Penaeus vannameiby-products and the bioactivity evaluation of the enzymatic hydrolysates

作　　者：吴泽龙 何建林 白锴凯 唐超 张怡[1,3] 洪碧红[2] WU Zelong;HE Jianlin;BAI Kaikai;TANG Chao;ZHANG Yi;HONG Bihong(Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition,Ministry of Education,Fuzhou 350002,China;Technical Innovation Center for Utilization of Marine Biological Resources,Third Institute of Oceanography,MNR,Xiamen 361005,China;College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]闽台特色海洋食品加工及营养健康教育部工程研究中心,福建福州350002 [2]自然资源部第三海洋研究所海洋生物资源开发利用工程技术创新中心,福建厦门361005 [3]福建农林大学食品科学学院,福建福州350002

出　　处：《应用海洋学学报》2023年第3期495-506,共12页Journal of Applied Oceanography

基　　金：自然资源部第三海洋研究所基本科研业务费(2018013);福建省海洋经济发展专项基金(FJHJF-L-2022-6)。

摘　　要：为高质化利用南美白对虾加工副产物蛋白资源,本研究以南美白对虾加工副产物虾头虾壳为原料,蛋白水解度为考察指标,采用单因素和响应面试验优化酶解工艺。检测虾蛋白肽分子量的分布范围,评价其抗氧化能力及免疫调节作用。结果表明,碱性蛋白酶与虾头内源酶自溶酶解具有较好的协同作用,最佳酶解工艺参数值为:酶添加量4%、酶解时间6.5 h、酶解温度40℃、料液比1∶5。经蛋白层析仪检测,分子质量分布在75~1 355 Da的蛋白肽占比达到76.35%。虾蛋白肽的羟自由基清除率IC50为4.64 mg/mL、DPPH自由基清除率IC50为3.08 mg/mL,提示其具有一定的抗氧化能力。虾蛋白肽中剂量组(10 mg/kg)能显著促进淋巴细胞增殖,并提高免疫低下模型小鼠的免疫器官指数和吞噬指数,表明虾蛋白肽具有显著的免疫调节作用。To utilize the protein resources of Penaeus vannamei by-products in a high-quality way,the enzymatic hydrolysis process of the by-product mainly shrimp heads and shells of Penaeus vannamei,was optimized by single-factor and response surface methodology enzymes using proteolytic degree as evaluation indicator.The molecular weight distribution range of shrimp peptides was detected and the antioxidant capacity and immune regulation activity evaluated.Results showed that Alkaline protease has a good synergistic effect with the endogenous enzymes in shrimp head and the optimized enzymatic process parameters were 4%of enzyme addition,6.5 h of enzymatic digestion time,40℃of enzymatic digestion temperature and 1∶5 of feed-to-liquid ratio.The molecular mass of shrimp peptide was mainly distributed in range of 75~1355 Da,accounting for 76.35%detected by gel chromato-graphy.The shrimp peptide had good scavenging activities on hydroxyl radical and 2,2-diphenyl-l-picrylhydrazyl(DPPH)with IC 50 values of 4.64 and 3.08 mg/mL,respectively.In addition,at dose of 10 mg/kg,shrimp peptide can significantly promote the proliferation of lymphocytes and improve the immune organ index and phagocytosis index of the immunocompromised model mice,exhibiting a significant immunomodulatory effect.

关 键 词：海洋生物学 南美白对虾 酶解 蛋白水解度 蛋白肽 抗氧化 免疫调节 

分 类 号：P735[天文地球—海洋生物学] P745[天文地球—海洋科学]