超微冷冻前处理协同渗漉法提取食叶草黄酮工艺优化及其抗氧化、降血糖活性研究  

Optimization of extraction process of flavonoids from Rumex patientia L.×Rumex tianschanicus A.LOS by ultramicro freezing pretreatment assisted percolation method and study its antioxidant and hypoglycemic activity

作　　者：孟楠 秦令祥 曹源 高愿军 MENG Nan;QIN Ling-Xiang;CAO Yuan;GAO Yuan-Jun(Luohe Vocational College of Food,Luohe 462300,China;Luohe Food Research Institute Co.,Ltd.,Luohe 462300,China;Henan Hesheng Food Co.,Ltd.,Luohe 462300,China;School of Food and Biological Engineering,Zhengzhou University of Light Industry,Zhengzhou 450002,China)

机构地区：[1]漯河食品职业学院,漯河462300 [2]漯河市食品研究院有限公司,漯河462300 [3]河南和生食品有限公司,漯河462300 [4]郑州轻工业大学食品与生物工程学院,郑州450002

出　　处：《食品安全质量检测学报》2023年第13期249-257,共9页Journal of Food Safety and Quality

基　　金：河南省高等学校重点科研项目(22B630008)。

摘　　要：目的优化食叶草黄酮的提取工艺,并对其体外抗氧化和降血糖活性进行研究。方法采用超微冷冻前处理协同渗漉法提取食叶草黄酮,通过单因素和响应面实验优化提取工艺参数。同时研究其对1,1-二苯基-2-苦基肼(1,1-diphenyl-2-bitter hydrazine,DPPH)自由基和2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐[2,2’-azino-bis-(3-ethylbenzothi-azoline-6-sulphonic acid),ABTS]阳离子自由基的清除能力和对α-葡萄糖苷酶活性的抑制率。结果最佳提取工艺条件为:乙醇浓度70%、溶剂倍数20、渗漉流速3.5 mL/min,此条件下,食叶草黄酮得率为3.58%,比普通粉碎法制得的得率提高了24.74%。体外抗氧化活性实验结果表明:当食叶草黄酮质量浓度为1 mg/mL时,其对DPPH自由基和ABTS阳离子自由基的清除率分别为76.27%和83.12%,半抑制浓度值(half maximal inhibitory concentration,IC_(50))分别为0.416 mg/mL和0.267 mg/mL,表明食叶草黄酮具有较强的抗氧化作用。体外降血糖实验结果表明,当食叶草黄酮质量浓度为5 mg/mL时,其对α-葡萄糖苷酶活性的抑制率为81.27%,IC_(50)为2.116 mg/mL,表明食叶草黄酮对α-葡萄糖苷酶有较好的抑制作用。结论该研究获得了食叶草黄酮的最佳提取工艺,得到的食叶草黄酮具有较强的抗氧化能力和降血糖能力。Objective To optimize the extraction process of flavonoids from Rumex patientia L.×Rumex tianschanicus A.LOS and study its antioxidant and hypoglycemic activity.Methods The ultramicro freezing grinding pretreatment assisted percolation method was used to extract flavonoids from Rumex patientia L.×Rumex tianschanicus A.LOS.And the extraction process parameters were optimized through single factor and response surface tests.At the same time,its ability to scavenge 1,1-diphenyl-2-bitter hydrazine(DPPH)free radicals and 2,2’-azino-bis-(3-ethylbenzothi-azoline-6-sulphonic acid)(ABTS)cation free radicals and its inhibition rate ofα-glucosidase activity had been studied.Results The optimum extraction conditions were:The ethanol concentration was 70%,the solvent ratio was 20,and the flow rate of percolation was 3.5 mL/min.Under these conditions,the yield of flavonoids from Rumex patientia L.×Rumex tianschanicus A.LOS was 3.58%,which was 24.74%higher than that obtained by ordinary crushing method.The results of antioxidant activity test showed that when the mass concentration of flavonoids flavonoids from Rumex patientia L.×Rumex tianschanicus A.LOS was 1 mg/mL,its scavenging rates of DPPH free radicals and ABTS cation free radicals were 76.27%and 83.12%,respectively,and its half maximal inhibitory concentration(IC_(50))was 0.416 mg/mL and 0.267 mg/mL,indicating that edible dock flavonoids had strong antioxidant effects.The results of hypoglycemic test showed that when the mass concentration of flavonoids from Rumex patientia L.×Rumex tianschanicus A.LOS was 5 mg/mL,its impact on the inhibition rate ofα-glucosidase activity was 81.27%,and the IC_(50) was 2.116 mg/mL,indicating that the flavonoids from Rumex patientia L.×Rumex tianschanicus A.LOS had a good inhibitory effect on the activity ofα-glucosidase.Conclusion The study has obtained the best extraction process of flavonoids from Rumex patientia L.×Rumex tianschanicus A.LOS,and which has strong antioxidant and hypoglycemic abilities.

关 键 词：食叶草 黄酮 超微冷冻 渗漉法 抗氧化活性 降血糖活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]