QuEChERS-气相色谱-串联质谱法检测软体类中18种酚类化合物  

Determination of 18 Kinds of Phenolic Compounds in Mollusk Aquatic Products by Gas Chromatography-Tandem Mass Spectrometry Coupled with QuEChERS

作　　者：邢家溧 李杨 郑睿行 徐晓蓉 毛玲燕 胡陵 沈坚 张书芬 承海 Xing Jiali;Li Yang;Zheng Ruihang;Xu Xiaorong;Mao Lingyan;Hu Ling;Shen Jian;Zhang Shufen;Cheng Hai(Ningbo Academy of Product and Food Quality Inspection(Ningbo Fibre Inspection Institute),Ningbo 315048,Zhejiang;College of Biological and Environmental Sciences,Zhejiang Wanli University,Ningbo 315100,Zhejiang;College of Science,Ningbo University of Technology,Ningbo 315211,Zhejiang;Zhejiang Fangyuan Testing Group,Hangzhou 310013)

机构地区：[1]宁波市产品食品质量检验研究院(宁波市纤维检验所),浙江宁波315048 [2]浙江万里学院生物与环境学院,浙江宁波315100 [3]宁波工程学院理学院,浙江宁波315211 [4]浙江方圆检测集团股份有限公司,杭州310013

出　　处：《中国食品学报》2023年第6期367-377,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家市场监管总局科技计划项目(2020MK117);浙江省市场监管系统科技计划项目(20210140,CY2023322);浙江省基础公益研究计划项目(LGC20C200013);宁波市自然科学基金项目(202003N4196,2019A610438,2019A610437);宁波市泛3315创新团队项目(2018B-18-C);宁波市高新精英创新团队项目(甬高科[2018]63号);宁波市公益类科技计划项目(2021S193);宁波市重大科技攻关项目(2021ZDYF020179)。

摘　　要：因目前国内外关于水产品中多种类酚类化合物的检测标准较为欠缺,且现有检测方法不能同时检测多种酚类化合物,故研究同时检测水产品中多种类酚类化合物的方法。以鱿鱼为空白样品,采用改良的QuEChERS前处理方法,对质谱条件、提取剂和提取剂用量等条件进行优化,同时采用响应面Box-Behnken方法优化净化剂。结果表明,18种酚类化合物测定方法的检出限为0.001~0.012 mg/kg,加标回收率83.1%~107.7%,相对标准偏差1.9%~9.5%。将建立的方法用于实际样品的检测,结果软体类水产品中均有酚类化合物检出,其中,扇贝和缢蛏的酚类污染最严重,牡蛎、贻贝和海参次之,鱿鱼污染程度最轻。本方法的建立为国家制定酚类化合物检测标准及水产品的安全监管提供技术参考。A method for the simultaneous determination of multiple phenolic compounds in aquatic products was investigated because there is a lack of standards for the detection of multiple phenolic compounds in aquatic products at home and abroad,and the existing detection methods cannot detect multiple phenolic compounds at the same time.A modified QuEChERS pretreatment method was used to optimize the mass spectrometry conditions,extractant and extractant dosage,and the response surface Box-Behnken method was used to optimize the clean-up agent.The results showed that the detection limits of the method for the determination of 18 phenolic compounds were in the range of 0.001-0.012 mg/kg,the spiked recoveries were 83.1%-107.7%and the relative standard deviations were 1.9%-9.5%.The method was applied to the actual samples and the results showed that phenolic compounds were detected in all molluscs,with scallops and razor clams showing the most serious phenolic contamination,followed by oysters,mussels and sea cucumbers,and squid the least contaminated.The method was developed to provide a technical reference for the development of national standards for the detection of phenolic compounds and the safety regulation of aquatic products.

关 键 词：气相色谱-串联质谱 QUECHERS 酚类化合物 水产品 检测 

分 类 号：O657.63[理学—分析化学] TS254.7[理学—化学]