莲藕多糖和阿魏酸的非共价相互作用及其复合物特性  

Non-covalent Interactions between Lotus Root Polysaccharides and Ferulic Acidand Their Complex Properties

作　　者：刘秋兰 邹晓琴 易阳 王宏勋[3] 孙莹[1,2] Liu Qiulan;Zou Xiaoqin;Yi Yang;Wang Hongxun;Sun Ying(School of Food Science and Engineering,Wuhan Polytechnic University,Wuhan 430023;Hubei Key Laboratory for Processing and Transformation of Agricultural Products,Wuhan Polytechnic University,Wuhan 430023;School of Life Science and Technology,Wuhan Polytechnic University,Wuhan 430023)

机构地区：[1]武汉轻工大学食品科学与工程学院,武汉430023 [2]武汉轻工大学农产品加工与转化湖北省重点实验室,武汉430023 [3]武汉轻工大学生命科学与技术学院,武汉430023

出　　处：《中国食品学报》2023年第7期26-36,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：湖北省重点研发计划项目(2022BBA0023);武汉市知识创新专项基础研究项目(2022020801010389);湖北省青年拔尖人才培养计划项目。

摘　　要：研究莲藕多糖与阿魏酸的非共价相互作用,及其复合物的理化和功能特性。以阿魏酸的结合量为指标,结合单因素实验和响应面试验优化复合物的制备条件。结果表明,在温度0℃、pH 7及多糖-阿魏酸质量比1∶2的条件下,低速搅拌30min,多糖的阿魏酸结合量达110.22 mg/g,其平均分子质量由191.8 ku增至297.4 ku。考虑各因素对阿魏酸结合量的影响,结合复合物的光谱分析发现,莲藕多糖与阿魏酸的非共价作用以氢键为主。低温有利于维持复合物的稳定,而复合物经口腔、胃或小肠模拟消化后的阿魏酸解离率均低于25%。相比莲藕多糖,复合物具有更强的DPPH自由基清除活性(P<0.05),而二者的巨噬细胞NO生成刺激能力无显著差异(P>0.05)。研究结果为改善莲藕多糖的功能活性和阿魏酸的生物利用率提供思路。This work aimed to investigate the non-covalent interaction between lotus root polysaccharide(LRP)and ferulic acid(FA),as well as the physicochemical and functional properties of LRP-FA complex.Based on single factor experiment and response surface experiment using the FA binding amount as evaluation index,the preparation conditions of LRP-FA complex were optimized.The results showed that,after 30 min low-speed mixing at temperature 0℃and pH 7 with the FA-LRP mass ratio of 1∶2,the binding amount of FA to LRP was 110.22 mg/g.The interaction increased the average molecular weight of LRP from 191.8 ku to 297.4 ku.According to the effect of experimental factors on the FA binding amount and the spectral analysis of LRP-FA complex,it was found that the non-covalent interaction between LRP and FA mainly depended on hydrogen bonding.Low temperature was beneficial to maintain the stability of LRP-FA complex,and its FA dissociation rate was lower than 25%by the simulated digestion of mouth,stomach and small intestine.Tthe complex had a better DPPH free radical scavenging activity compared with LRP(P<0.05),however,their stimulating abilities on the NO production of macrophages showed no significant difference(P>0.05).The results provide ideas for improving the functional activity of LRP and the bioavailability of FA.

关 键 词：莲藕多糖 阿魏酸 非共价复合物 稳定性 活性 

分 类 号：TS254.58[轻工技术与工程—水产品加工及贮藏工程]