板栗壳色素通过AMPK信号通路改善肥胖小鼠的脂代谢紊乱  

Chestnut Shells Pigment Ameliorated Lipid Metabolism Disorder in Obese Micethrough AMPK Signaling Pathway

作　　者：张晓云 梅晓宏[1,2] Zhang Xiaoyun;Mei Xiaohong(College of Food Science and Nutrition Engineering,China Agricultural University,Beijing 100083;Key Laboratory of Safety Assessment of Genetically Modified Organism(Food Safety),Ministry of Agriculture and Rural Affairs,Beijing 100083)

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]农业农村部农业转基因生物安全评价(食用)重点实验室,北京100083

出　　处：《中国食品学报》2023年第7期177-186,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家科技重大专项(2018ZX0801102B-002)。

摘　　要：目的:探讨板栗壳色素(CSP)对肥胖小鼠脂代谢紊乱的改善作用及其分子机制。方法:将雄性C57BL/6J小鼠分为正常饮食(N)组、高脂饮食(HFD)组、高脂饮食+20 mg/kg CSP(HFD-L)组、高脂饮食+50 mg/kg CSP(HFD-H)组、高脂饮食+20mg/kg儿茶素(HFD-C)组,每周测量小鼠体质量和进食量,14周后检测小鼠肝脏和附睾脂肪质量、血清血脂水平,进行脂肪与肝脏形态学分析,检测肝脏中脂肪代谢关键蛋白的表达等。结果:与HFD组相比,HFD-L和HFD-H组小鼠体质量增长量分别降低28.67%和39.16%;附睾脂肪质量分别降低23.63%和42.80%;CSP能减少进食量、脂肪细胞的扩大和肝脏脂肪的积累,降低血清TG、TC、LDL-C、ALT和AST水平,升高HDL-C,显著升高小鼠肝脏组织中p-AMPK/AMPK、p-ACC/ACC、PPARα的表达量,显著降低SREBP-1c和HMGCR的表达量,且作用效果比单一儿茶素效果好。结论:板栗壳色素具有改善肥胖小鼠脂代谢的作用,此作用与激活AMPK信号通路相关。Objective:To investigate the improvement effect of chestnut shells pigment(CSP)on lipid metabolism disor der in obese mice and its molecular mechanism.Methods:Male C57BL/6J mice were divided into normal diet group(N),high fat diet group(HFD),high fat diet+20 mg/kg CSP group(HFD-L),high fat diet+50 mg/kg CSP group(HFD-H),high fat diet+20 mg/kg catechin group(HFD-C).The body weight and food intake of mice were measured every week.After 14 weeks,the weight of liver and epididymis fat of mice and lipid levels of serum were detected.The morphology of fat and liver was analyzed and the expression of key proteins of fat metabolism in liver was detected.Results:Compared with HFD group,the gain of body mass in HFD-L and HFD-H groups decreased by 28.67%and 39.16%,respectively.Fat mass of epididymis decreased by 23.63%and 42.80%,respectively.CSP can reduced food intake and accumulation of liver fat,reduced serum TG,TC,LDL-C,ALT and AST levels,increased HDL-C.The expression levels of p-AMPK/AMPK,p-ACC/ACC and PPARαin liver tissue were significantly increased,however,the expression levels of SREBP-1C and HMGCR were significantly decreased,which were better than those of pure catechin.Conclusion:CSP can improve lipid metabolism in obese mice,which is related to the activation of AMPK signaling pathway.

关 键 词：板栗壳色素 高脂饮食 肥胖 脂代谢 腺苷酸活化蛋白激酶 

分 类 号：TS209[轻工技术与工程—食品科学] TS201.4[轻工技术与工程—食品科学与工程]