高山被孢霉低温应激条件下油脂代谢研究  

Study on lipids metabolism of Mortierella alpina at low temperatures stress

作　　者：刘雯茜 王海军[2,3] 陈吴西[2] 李德茂[2] 刘秀敏[4] 李军[1] LIU Wen-qian;WANG Hai-jun;CHEN Wu-xi;LI De-mao;LIU Xiu-min;LI Jun(College of Food Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao 066600,Hebei,China;Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China;College of Biological Engineering,Tianjin University of Science and Technology,Tianjin 300308,China;Grain Engineering Research and Development Center,Hebei Jiaotong Vocational and Technical College,Shijiazhuang 050035,Hebei,China)

机构地区：[1]河北科技师范学院食品科技学院,河北秦皇岛066600 [2]中国科学院天津工业生物技术研究所,天津300308 [3]天津科技大学生物工程学院,天津300308 [4]河北交通职业技术学院粮食工程研发中心,河北石家庄050035

出　　处：《粮食与油脂》2023年第8期46-51,共6页Cereals & Oils

基　　金：国家重点研发计划(2019YFA0904900);河北省果品加工技术创新中心绩效补助经费项目(225676115H)。

摘　　要：对高山被孢霉在不同温度下的生物量、油脂产量和油脂组成等指标进行研究,并开展了比较代谢组学研究。结果表明:在低温处理(15℃)时,高山被孢霉的生物量和油脂含量均低于常温(25℃)处理。但是,在低温条件下高山被孢霉的极性脂含量提高到了2.53倍,而且极性脂中的不饱和脂肪酸含量提高到了1.32倍,表明高山被孢霉的低温保护机制启动,大量合成极性脂和不饱和脂肪酸用于抵御低温应激。差异化合物筛选发现其中与油脂合成和降解有关的有10个化合物,与糖酵解(EMP)途径有关的有6个化合物,与氨基酸合成、降解和转运相关的化合物有5个。The biomass,oil yield and oil composition of Mortierella alpina at different temperatures were studied,and the comparative metabolomics was carried out.The results showed that the biomass and oil content of Mortierella alpina were lower at low temperature(15 C)than at normal temperature(25 C).However,the content of polar lipids increased to 2.53 times at low temperature,and the content of unsaturated fatty acids in polar lipids increased to 1.32 times at low temperature,indicating that the low temperature protection mechanism was activated,and a large number of polar lipids and unsaturated fatty acids were synthesized to resist low temperature stress.Differential compound screening showed that 10 compounds were related to lipid synthesis and degradation,6 compounds were related to glycolysis(EMP)pathway,and 5 compounds were related to amino acid synthesis,degradation and transport.

关 键 词：高山被孢霉 油脂 代谢组学 中性脂 极性脂 

分 类 号：TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]