梅卤中多糖的分离纯化及其结构的初步解析  

Isolation,purification and preliminary structure analysis of polysaccharide from plum bittern

作　　者：梁诗洋 张鹰[1,2,3,4] 曾晓房 许佳木[5] 林海滨 白卫东 LIANG Shi-yang;ZHANG Ying;ZENG Xiao-fang;XU Jia-mu;LIN Hai-bin;BAI Wei-dong(College of Light Industry and Food Science,Zhongkai University of Agricultural and Engineering,Guangzhou 510225,Guangdong,China;Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,Guangdong,China;Academy of Contemporary Agricultural Engineering Innovations,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,Guangdong,China;Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food,Ministry of Agriculture and Rural Affairs,Guangzhou 510225,Guangdong,China;Guangdong Dianyutian Food Co.,Ltd.,Jieyang 515332,Guangdong,China)

机构地区：[1]仲恺农业工程学院轻工食品学院,广东广州510225 [2]仲恺农业工程学院广东省岭南特色食品科学与技术重点实验室,广东广州510225 [3]仲恺农业工程学院现代农业研究院,广东广州510225 [4]农业农村部岭南特色食品绿色加工与智能制造重点实验室,广东广州510225 [5]广东殿宇田食品有限公司,广东揭阳515332

出　　处：《粮食与油脂》2023年第9期111-115,122,共6页Cereals & Oils

基　　金：教育部农业部科协资助项目“广东普宁青梅科技小院”;广东省科技厅&广东省农业农村厅项目(KTP20210194);仲恺农业工程学院-广东丰澍工程咨询有限公司联合培养研究生基地(KA220160259)。

摘　　要：对梅卤多糖(PPM)进行了分离纯化,对梅卤多糖组分(PPM-Ⅱa)进行纯度分析及结构鉴定。利用DEAE-52纤维素阴离子柱层析法对PPM进行分离纯化得到4个组分,即PPM-Ⅰ、PPM-Ⅱ、PPM-Ⅲ和PPM-Ⅳ;经过凝胶渗透色谱筛选出PPM-Ⅱ组分为均一组分作为进一步纯化的对象;PPM-Ⅱ通过Sephadex G-100凝胶色谱柱纯化得到PPM-Ⅱa。PPM-Ⅱa主要是半乳糖、半乳糖醛酸和阿拉伯糖组成的酸性多糖,其含量分别为332832.01、25099.18、22801.19 mg/kg;经红外光谱与核磁共振氢谱判断,PPM-Ⅱa是含有β糖苷键的吡喃环酸性多糖,且刚果红试验结果表明PPM-Ⅱa存在三螺旋结构。The polysaccharides from plum bittern(PPM)was isolated and purified.The purity of plum bittern poly saccaride component(PPM-II a)was analyzed and its structure was identified.Four components,PPM-I,PPM-II,PPM-IIand PPM-IV,were isolated and purified by DEAE-52 cellulose anion column chromatography.After gel permeation chromatography,PPM-II was selected as a homogeneous component for further purification.PPM-II a was purified by Sephadex G-100 gel column.PPM-II a was mainly an acidic polysaccharide composed of galactose,galacturonic acid and arabinose,and its contents were 332832.01,25099.18 and 22801.19 mg/kg,respectively.According to the infrared spectrum and nuclear magnetic resonance hydrogen spectrum,PPM-II a was a pyranoic acid polysaccharide contai-ningβglycosidic bonds,and the Congo red test results showed that PPM-IIa had triple helix structure.

关 键 词：梅卤 多糖 分离 纯化 结构鉴定 

分 类 号：TS201.1[轻工技术与工程—食品科学]