体外模拟消化对抹茶冲泡液生物活性物质组成及抗氧化活性的影响  

Effects of simulated digestion in vitro on the bioactive substance composition and antioxidant activity of the brewing Matcha tea

作　　者：崔虹[1] 黄帅 刘宝林[2] CUI Hong;HUANG Shuai;LIU Baolin(College of Environmental Engineering,Yellow River Conservancy Technical Institute,Kaifeng 475004;College of Chemistry and Chemical Engineering,Henan University,Kaifeng 475004)

机构地区：[1]黄河水利职业技术学院环境工程学院,开封475004 [2]河南大学化学化工学院,开封475004

出　　处：《中国食品添加剂》2023年第9期113-119,共7页China Food Additives

基　　金：河南省高等学校重点科研项目计划(21A170015)。

摘　　要：为探明消化过程对抹茶冲泡液中生物活性物质和抗氧化活性的影响,采用体外消化模型方法,研究各消化阶段(口腔、胃和肠)中总黄酮、总多酚、茶氨酸、咖啡碱、儿茶素类化合物和抗氧化活性的变化。结果表明,与口腔消化阶段相比,在胃消化后,抹茶冲泡液中总多酚和茶氨酸分别显著提高41.84%和19.54%(P<0.05);绿原酸、没食子酸、儿茶素、表儿茶素、表没食子儿茶素和表没食子儿茶素没食子酸酯分别显著提高38.53%、44.75%、13.48%、40.45%、64.49%和67.34%(P<0.05);而DPPH和ABTS+自由基清除率分别显著增加13.74%和9.56%(P<0.05)。与胃消化阶段相比,在肠消化后,这些生物活性物质和抗氧化活性显著降低(P<0.05)。相关性结果表明酚酸和儿茶素类化合物是抹茶冲泡液中主要的抗氧化活性物质。To investigate the effects of digestion process on the bioactive substances and antioxidant activity in Matcha tea,the in vitro digestion model was used to study the total flavonoids,total polyphenols,theanine,caffeine,catechin compounds and antioxidant activities in each digestion stage(oral,gastric and intestinal digestion).Compared with oral digestion,gastric digestion was very powerful:the total polyphenols and theanine in the Matcha tea were significantly increased by 41.84%and 19.54%respectively(P<0.05),and chlorogenic acid,gallic acid,catechin,epicatechin,epigallocatechin and epigallocatechin gallate were significantly increased by 38.53%,44.75%,13.48%,40.45%,64.49% and 67.34%,respectively(P<0.05);the free radical scavenging rates of DPPH and ABTS were also significantly increased by 13.74% and 9.56%,respectively(P<0.05).Compared with the stage of gastric digestion,these bioactive substances and antioxidant activities were significantly decreased(P<0.05)after intestinal digestion.The correlation results showed that phenolic acids and catechins were the main antioxidant active substances in the brewing Matcha tea.

关 键 词：抹茶 冲泡液 体外模拟消化 生物活性物质 抗氧化活性 

分 类 号：TS272.7[农业科学—茶叶生产加工]