响应面法优化超声波富集青稞酚类物质工艺研究  

Optimization of Ultrasonic Enrichment Process of Phenol in Highland Barley by Response Surface Methodology

作　　者：张杰[1,2,3] ZHANG Jie(Academy of Agriculture and Forestry Sciences,Qinghai University,Xining Qinghai 810016,China;Qinghai Tibetan Plateau Key Laboratory of Agricultural Product Processing,Xining Qinghai 810016,China;Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources,Xining Qinghai 810016,China)

机构地区：[1]青海大学农林科学院,青海西宁810016 [2]青海省青藏高原农产品加工重点实验室,青海西宁810016 [3]青藏高原种质资源研究与利用实验室,青海西宁810016

出　　处：《青海农林科技》2023年第3期77-83,共7页Science and Technology of Qinghai Agriculture and Forestry

基　　金：青海省科技基础条件平台(2022-ZJ-T04);国家重点研发计划项目(2022YFD2301300)。

摘　　要：本研究以青稞为原料,以总酚含量为指标,采用单因素试验及Box-Behnken响应面设计优化超声波处理富集青稞多酚的最佳工艺参数,并在此基础上比较超声波处理前后青稞的总酚含量及抗氧化活性差异。结果表明,超声波处理富集青稞多酚的最优工艺为:超声温度35℃,超声时间30 min,超声功率500 W,在此条件下青稞中总酚含量为636.15 mg/100 g。抗氧化活性结果表明,经超声处理后的青稞其DPPH·清除能力及FRAP还原能力均显著高于未处理样品。该研究可为进一步提高青稞的利用价值提供参考。This study was based on highland barley as the raw material.The content of total phenolic were used as indicators to determine the optimal process parameters of ultrasonic treatment for the enrichment of highland barley polyphenols by the single factor test and Box-Behnken response surface design.Then the total phenol content and antioxidant activity of black barley were compared before and after ultrasonic treatment.The results showed that the optimal conditions of ultrasonic pretreatment were as follows:ultrasonic treatment temperature 35℃,ultrasonic treatment time 30 min,ultrasonic power 500 W.Under these conditions,the content of the total phenol content reached 636.15 mg/100 g.The results of antioxidant activity showed that the DPPH·scavenging capacity and FRAP reducing capacity of ultrasonically treated barley were significantly higher than those of untreated samples.This study could provide a reference for further improving the utilization value of highland barley.

关 键 词：青稞 多酚 响应面 超声波处理 

分 类 号：TS210.4[轻工技术与工程—粮食、油脂及植物蛋白工程]