硒化甜玉米芯多糖对非酶糖基化的抑制作用  

Inhibitory Effect of Selenium Sweet Corncob Polysaccharide on Non-Enzymatic Glycosylation

作　　者：王鑫[1] 周卓 王峙力 修伟业 罗钰 马永强[1] WANG Xin;ZHOU Zhuo;WANG Zhili;XIU Weiye;LUO Yu;MA Yongqiang(Key Laboratory of Cereal Food and Cereal Resources in Heilongjiang Province,School of Food Engineering,Harbin University of Commerce,Harbin 150028,China)

机构地区：[1]黑龙江省谷物食品与谷物资源重点实验室/哈尔滨商业大学食品工程学院,黑龙江哈尔滨150028

出　　处：《食品工业科技》2023年第19期17-23,共7页Science and Technology of Food Industry

基　　金：哈尔滨商业大学“青年创新人才”支持计划项目(2019CX31);哈尔滨商业大学产业化项目(22CZ16)。

摘　　要：以甜玉米芯多糖(SCP)为原料采用咪唑介导法合成一种硒含量为(7.19±0.067)mg/g的硒化多糖(Se-SCP),为探究Se-SCP对非酶糖基化反应的抑制作用,本文选用了果糖、牛血清白蛋白(bovine serum albumin,BSA)为底物,建立清白蛋白/果糖模拟体系。对非酶糖基化过程中的前、中、末期三个特征产物果糖胺→α-二羰基化合物→非酶糖基化终产物(AGEs)进行测定并通过透射电子显微镜(TEM)和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(sodium dodecyl sulfate-polyacrylamide gel electrophoresis,SDS-PAGE)在微观结构方面对比分析原多糖、硒多糖对非酶糖基化进程的影响。结果表明,Se-SCP对蛋白非酶糖基化过程的三个阶段均有不同程度的抑制作用,对非酶糖基化过程中的特征产物抑制率分别达到了89.5%、74.9%与71.7%;在抑制蛋白质交联结构方面,Se-SCP与SCP组相比,纹理相对清晰,结构密度较小,抑制效果明显;SDS-PAGE结果表明,Se-SCP减少了果糖诱导的高分子量交联蛋白的形成,其中Se-SCP效果较好,与阳性药物效果相似。综上,Se-SCP具有良好的抗非酶糖基化活性,在抑制糖尿病及其并发症方面具有巨大潜力。A selenized polysaccharide(Se-SCP)with selenium content of(7.19±0.067)mg/g was synthesized by imidazole-mediated method using sweet corncob polysaccharide(SCP)as raw material.In order to explore the inhibitory effect of Se-SCP on non-enzymatic glycosylation reaction,fructose and bovine serum albumin(BSA)were selected as substrates to establish albumin/fructose simulation system.Fructosamine→α-dicarbonyl compounds→advanced glycation end products(AGEs),the three characteristic products in the non-enzymatic glycosylation process,were determined and analyzed by transmission electron microscopy(TEM)and sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE).The effects of protopolysaccharides and selenium polysaccharides on the non-enzymatic glycosylation process were compared and analyzed in terms of microstructure.The results showed that Se-SCP had different degrees of inhibition on the t hree stages of protein non-enzymatic glycosylation process,and the inhibition rates of characteristic products in non-enzymatic glycosylation process reached 89.5%,74.9%and 71.7%,respectively.In terms of inhibiting protein cross-linking structure,compared with the SCP group,the texture of Se-SCP was relatively clear,the structural density was small,and the inhibition effect was obvious.The results of SDS-PAGE showed that Se-SCP reduced the formation of high molecular weight cross-linked protein induced by fructose,and the effect of Se-SCP was better,which was similar to that of positive drug.In summary,Se-SCP has good anti-nonenzymatic glycation activity and has great potential in inhibiting diabetes and its complications.

关 键 词：甜玉米芯多糖 硒多糖 非酶糖基化 抑制作用 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]