同步糖化发酵甘蔗渣产乙醇的尖孢镰刀菌筛选及其产醇性能  

Screening of Fusarium oxysporum for Ethanol Production by Simultaneous Saccharification and Fermentation of Bagasse and Its Ethanol Production Performance

作　　者：湛佳佳 周泉 熊兴耀 张志旭[4,5] 胡亚平[1] 李璧[1] 陈宗瑞 龚奕杭 贺如男 秦丹 曾璐[1] ZHAN Jiajia;ZHOU Quan;XIONG Xingyao;ZHANG Zhixu;HU Yaping;LI Bi;CHEN Zongrui;GONG Yihang;HE Runan;QIN Dan;ZENG Lu(College of Food Science and Technology,Hunan Agricultural University,Changsha 410128,China;Medical&Technology School of Zunyi Medical University,Zunyi 563000,China;Agricultural Genome Research Institute,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China;College of Horticulture and Landscape,Hunan Agricultural University,Changsha 410128,China;Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding,Ministry of Education,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学食品科学技术学院,湖南长沙410128 [2]遵义医科大学医学与科技学院,贵州遵义563000 [3]中国农业科学院农业基因组研究所,广东深圳518120 [4]湖南农业大学园艺学院,湖南长沙410128 [5]湖南农业大学园艺作物种质创新与新品种选育教育部工程研究中心,湖南长沙410128

出　　处：《食品工业科技》2023年第19期108-116,共9页Science and Technology of Food Industry

基　　金：湖南省大学生创新创业训练计划项目(S202110537006);湖南省教育厅项目(19C0941);作物种质创新与资源利用重点实验室(省部共建国家重点实验室培育基地)科学基金开放项目(19KFXM08);云南特异辣椒资源高效利用研究(202202AE090031)。

摘　　要：为获得同步糖化发酵(Simultaneous saccharification and fermentation,SSF)产乙醇的优良尖孢镰刀菌菌株,对14株尖孢镰刀菌依次进行纤维素酶活力测定、刚果红平板染色、滤纸片崩解试验筛选,获得纤维素降解能力较强的菌株,进而以甘蔗渣为底物,进行SSF产乙醇试验,探究其纤维素降解及糖转化性能。结果表明:菌株mh2的纤维素降解能力较强,其纤维素酶活力达到14.28 U/mL,透明圈直径为4.5 cm,能使滤纸片完全降解成糊状;并且对含残糖及去糖的甘蔗渣均具有良好的同步糖化发酵产醇效果,乙醇得率分别可达98、41.7 g/kg,其乙醇转化率最高可达33.36%;此外mh2纤维素酶系统中的滤纸酶、内切葡聚糖酶、β-葡聚糖苷酶、外切葡聚糖酶活力分别达到25.63、15.69、19.61、22.44 U/mL,对甘蔗渣纤维素降解率达到25.6%,对葡萄糖和木糖的乙醇转化率分别可达0.443、0.213 g/g,分别是对照菌株Cs20降解率和葡萄糖、木糖乙醇转化率的1.58、1.17、3.8倍,表明mh2在SSF过程中具有良好的降解及糖转化性能。本研究可为丰富SSF菌株资源及单菌株利用木质纤维素生产乙醇的SSF研究提供数据参考。In order to obtain an excellent Fusarium oxysporum strain for simultaneous saccharification and fermentation(SSF),the cellulase activity of the 14 strains of Fusarium oxysporum was determined,and Congo red plate staining and paper disintegrating tests were carried out to screen the strains,the ethanol production experiment of SSF was carried out to investigate its cellulose degradation and sugar conversion performance.The results showed that strain mh2 had strong cellulose degradation ability,its cellulase activity was 14.28 U/mL,the diameter of transparent circle was 4.5 cm,which could completely degrade the filter paper into paste.The yield of ethanol could reach 98 g/kg and 41.7 g/kg,respectively,and the conversion of ethanol could reach up to 33.36%.In addition,the activities of filter paper enzyme,endo-glucanase,β-glucanase and exo-glucanase in mh2 cellulase system were 25.63,15.69,19.61 and 22.44 U/mL,respectively,the degradation rate of bagasse cellulose was 25.6%,and the ethanol conversion rate of glucose and xylose was 0.443 and 0.213 g/g,respectively,the biodegradability of mh2 was 1.58,1.17 and 3.8 times higher than that of Cs20,respectively,indicating that mh2 had good biodegradability and sugar conversion during SSF.This study can provide data for the study of SSF to enrich the resources of SSF strains and single strain to produce ethanol from lignocellulose.

关 键 词：甘蔗渣 尖孢镰刀菌 同步糖化发酵 纤维素乙醇 木糖 

分 类 号：TS209[轻工技术与工程—食品科学]