金盏菊乙酸乙酯萃取相的酚酮类物质含量及其抗氧化、抑菌和酪氨酸酶抑制活性  

Phenolic Ketone Contents and Antioxidant,Antibacterial Properties and Inhibitory Tyrosinase Activity of the Ethyl Acetate Extracted from Calendula officinalis L.

作　　者：吴颖 王双旦 唐文 刘晴 王佳其 张蓓婷 WU Ying;WANG Shuangdan;TANG Wen;LIU Qing;WANG Jiaqi;ZHANG Beiting(School of Chemical and Environmental Engineering,Shanghai Institute of Technology,Shanghai 201418,China;School of Perfume and Aroma Technology,Shanghai Institute of Technology,Shanghai 201418,China)

机构地区：[1]上海应用技术大学化学与环境工程学院,上海201418 [2]上海应用技术大学香料香精化妆品学部,上海201418

出　　处：《食品工业科技》2023年第19期347-355,共9页Science and Technology of Food Industry

摘　　要：本文研究了金盏菊乙酸乙酯萃取相(Ethyl acetate extraction,EA相)及其相关萃取相的总酚、总黄酮含量、体外抗氧化及抑菌活性,采用高效液相色谱(High performance liquid chromatography,HPLC)分析其中没食子酸、绿原酸、咖啡酸、芦丁和阿魏酸5种酚酮类物质的含量。结果表明,EA相的总酚酮含量、抗氧化和抑菌活性均高于其他萃取相,EA相总酚酮含量为330.760±4.640 mg/g,芦丁和没食子酸在EA相中含量最高,分别为0.978±0.203和0.230±0.301 mg/g;EA相抗氧化活性与阳性对照VC接近,对金黄色葡萄球菌属于中度敏感;EA相对酪氨酸酶活性有较好地抑制作用,其IC50为0.965±0.001 mg/mL,优于阳性对照熊果苷,其抑制作用表现为可逆的混合型抑制,抑制常数KI和KIS分别为0.254和5.577 mg/mL。EA相抗氧化、抑菌、抑制酪氨酸酶活性作用最好,与其总酚、总黄酮含量较高且富含功能成分有较大的相关性,这些酚酮类活性物质更易被乙酸乙酯溶剂所萃取。In this paper,the total phenolic and total flavonoid content,in vitro antioxidant and antibacterial activities of ethyl acetate extraction(EA phase)and its related extracted phases of Calendula officinalis L were studied.The content of five phenolic ketones,namely gallic acid,chlorogenic acid,caffeic acid,rutin and ferulic acid was analyzed by high performance liquid chromatography(HPLC).The results showed that the total phenolic ketone content,antioxidant and antibacterial activities of EA phase were higher than other extracted phases,the total phenolic ketone content of EA phase was 330.760±4.640 mg/g,rutin and gallic acid were the highest in EA phase,which were 0.978±0.203 and 0.230±0.301 mg/g,respectively.The antioxidant activity of EA phase was close to that of the positive control VC,and moderately sensitive to Staphylococcus aureus.The enzyme kinetic experiment showed that EA phase had a good inhibiting effect on tyrosinase activity with an IC50 of 0.965±0.001 mg/mL,which was better than that of the positive control arbutin.The results demonstrated that EA phase suppressed the activity of tyrosinase in a reversible mixed-inhibition,and the inhibition constants KI and KIS were 0.254 and 5.577 mg/mL,respectively.The best antioxidant,antibacterial and tyrosinase inhibiting effects of EA phase were correlated with the high content of total phenols and total flavonoids and their richness in functional components,which were more easily extracted by ethyl acetate solvent.

关 键 词：金盏菊 总黄酮 总酚 酪氨酸酶 抗氧化活性 

分 类 号：R15[医药卫生—营养与食品卫生学]