冷藏期间鲜切莲藕的微生物多样性变化规律分析  

Microbial Diversity Analysis of Fresh-Cut Lotus Root During Cold Storage

作　　者：张周未 何建军[1] 范传会 熊添 施建斌[1] 陈学玲[1] ZHANG Zhouwei;HE Jianjun;FAN Chuanhui;XIONG Tian;SHI Jianbin;CHEN Xueling(Key Laboratory of Cold Chain Logistics Technology for Agro-Product,Ministry of Agriculture and Rural Affairs,Institute of Agro-Products Processing and Nuclear Agricultural Technology,Hubei Academy of Agricultural Sciences,Wuhan 430064,China)

机构地区：[1]湖北省农业科学院农产品加工与核农技术研究所,农业农村部农产品冷链物流技术重点实验室,湖北武汉430064

出　　处：《食品科技》2023年第7期16-21,共6页Food Science and Technology

基　　金：国家重点研发计划项目(2018YFD0401301);湖北省农业科学院青年基金项目(2020NKYJJ15)。

摘　　要：为探究鲜切莲藕在冷藏期间微生物多样性的变化规律,明确鲜切莲藕冷藏期间的主要微生物种类及其生长规律,以4℃贮藏的鲜切莲藕为研究对象,分别对贮藏0、3、6、9、12 d的鲜切莲藕中真菌ITS和细菌16S rDNA序列进行高通量测序分析。结果表明,冷藏期间鲜切莲藕中微生物,共鉴定出真菌2个门、19个属,细菌2个门、5个属。在不同冷藏阶段,鲜切莲藕中的微生物存在差异。在属水平上,冷藏0 d时优势真菌为陶氏菌属,3~12 d冷藏阶段的优势真菌为长垣菌属,且长垣菌属在冷藏12 d时极显著富集;冷藏0~9 d优势细菌为不动杆菌属,而冷藏12 d时优势细菌为假单胞菌属,且假单胞菌属在冷藏12 d时极显著富集。通过研究初步明确了冷藏期间鲜切莲藕的微生物菌群结构和变化规律,为鲜切莲藕保鲜技术和质量控制研究提供参考。In order to explore the change rule of microbial diversity of fresh-cut lotus root,and clarify the main microbial species and growth rule during cold storage.The fresh-cut lotus root was stored at 4℃,and it’s sequences of ITS in fungi and 16S rRNA in bacteria were analyzed by high-throughput sequencing technology.The results showed that microbes in fresh-cut lotus root were identified as 2 phyla and 19 genera of fungi and 2 phyla and 5 genera of bacteria during cold storage.Microbes in fresh-cut lotus root were different in different cold storage stages.At the genus level,Tausonia was the dominant fungus at 0 d,while Naganishia became dominant at 3~12 d which was significantly enriched at 12 d.Acinetobacter was the dominant flora a 0~9 d,while Pseudomonas was the dominant bacteria and significantly enriched at 12 d.This study preliminarily clarified the structure and change rule of microbes of fresh-cut lotus root during cold storage,which provided references for preserving the fresh-cut lotus root and quality control.

关 键 词：鲜切莲藕 微生物多样性 冷藏 ITS 16S rDNA 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]