Calycosin attenuates the inflammatory damage of microglia induced by oxygen and glucose deprivation through the HMGB1/TLR4/NF-κB signaling pathway  

作　　者：Xiang Li Xin Yang Huiling Lu Wenbo Wang Le Cai Jian Chen Yong Wang 

机构地区：[1]Key Laboratory of Tumor Immunology and Microenvironmental Regulation,Guilin Medical University,Guilin,541199,China [2]Department of Pathology and Physiopathology,Guilin Medical University,Guilin 541199,China [3]Department of Neurosurgery,Nanxishan Hospital of Guangxi Zhuang Autonomous Region,Guilin 541002,China [4]Department of Physiology,Guilin Medical University,Guilin 541199,China

出　　处：《Acta Biochimica et Biophysica Sinica》2023年第9期1415-1424,共10页生物化学与生物物理学报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China(Nos.81860231 and 82260711);the Guangxi Natural Science Foundation(Nos.2018GXNSFAA050054 and 2018GXNSFAA294138);the Scientific Research Ability Enhancement Project for Young and Middle-aged Faculty of Guilin Medical University(No.20502018006).

摘　　要：Stroke seriously threatens human life and health worldwide,but only very few effective stroke medicines are currently available.Our previous studies have indicated that the phytoestrogen calycosin exerts neuroprotective effects in cerebral ischemia and reperfusion injury rats.Therefore,the objective of this study is to further explore the protective effect of calycosin on inflammatory injury in microglia after oxygen-glucose deprivation/reoxygenation(OGD/R)and to clarify whether its protective effect is related to the HMGB1/TLR4/NF-κB signaling pathway.Here,the OGD/R model of rodent microglia is established in vitro to simulate cerebral ischemia-reperfusion injury.Through the CCK-8 test,ELISA,qRT-PCR,and western blot analysis,we find that the activity of microglia is decreased,the expressions of HMGB1 and TLR4 and the phosphorylation of NF-κB(p-NF-κB)are increased,and the releases of the inflammatory factors IL-6,IL-1β,and TNF-αare increased after OGD/R.Pretreatment with calycosin could ameliorate these states,increase cell viability,reduce HMGB1,TLR4 and p-NF-κB expression,and reduce inflammatory cytokine production.In addition,the effect of calycosin is similar to that of TAK-242(an inhibitor of TLR4),and the effect of the combined treatment is better than that of the single treatment.The results indicate that calycosin protects microglia from OGD/R injury and reduces the inflammatory response.Calycosin might alleviate cerebral ischemiareperfusion injury by inhibiting the HMGB1/TLR4/NF-κB pathway.

关 键 词：CALYCOSIN HMGB1/TLR4/NF-κB signaling pathway MICROGLIA oxygen and glucose deprivation NEUROINFLAMMATION 

分 类 号：R74[医药卫生—神经病学与精神病学]