氨基酸含量结合多元统计分析对蜂蜜品种的判别  

Discrimination of Botanical Origins for Chinese Honey According to Amino Acids Content with Chemometric Approaches

作　　者：问亚琴 张金振[2,3] 罗帅 WEN Yaqin;ZHANG Jinzhen;LUO Shuai(Beijing Polytechnic,College of Biotechnology,Beijing 100176,China;State Key Laboratory of Resource Insects,Bee Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100093,China;Bee Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100093,China)

机构地区：[1]北京电子科技职业学院生物工程学院,北京100176 [2]资源昆虫高效养殖与利用全国重点实验室,中国农业科学院蜜蜂研究所,北京100093 [3]中国农业科学院蜜蜂研究所,北京100093

出　　处：《食品工业科技》2023年第20期305-310,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金(31701669);北京市教育委员会科学研究计划项目资助(KM201910858003);北京市科学技术协会青年人才托举工程(BYESS2022135);北京市属高等学校优秀青年人才培育计划项目(BPHR202203242)。

摘　　要：本研究利用氨基酸分析仪对我国3个不同品种单花蜜洋槐蜜、椴树蜜和油菜蜜,共计110个蜂蜜样品中17种氨基酸含量进行分析,并基于氨基酸含量结合统计分析方法进行蜂蜜种类鉴别分析。结果表明,油菜蜜中水解氨基酸含量高于椴树蜜和洋槐蜜,16种氨基酸含量在三个蜂蜜品种间存在差异。主成分分析(principle component analysis,PCA)结果表明不同植物源蜂蜜具有聚类趋势,偏最小二乘法判别分析(Partial least squares discriminant analysis,PLS-DA)结果表明油菜蜜可以和洋槐蜜和椴树蜜区分开来。线性判别分析(Linear discriminant analysis,LDA)结果表明3种蜂蜜整体判别率为92.7%,油菜蜜的判别率为92.3%。本研究为油菜蜜、洋槐蜜和椴树蜜分类鉴别提供数据支撑和参考依据。In this study,17 amino acids were analyzed by amino acid analyzer in 110 honey samples from three different monofloral honeys,i.e.acacia honey,linden honey and rape honey in China,and the honey variety discrimination was performed based on amino acid content combined with chemometrics.The results showed that the content of hydrolyzed amino acids was higher in rape honey than in acacia and linden honey,and the content of 16 amino acids showed significant differences among the honey varieties.The results of principal component analysis(PCA)revealed that the botanical origins of honey samples clustered according to their amino acid content.The results of least squares discriminant analysis(PLS-DA)showed that rape honey could be distinguished from acacia honey and linden honey.Linear discriminant analysis(LDA)showed that the overall discriminant rate of the three honeys was 92.7%,and the discriminant rate of rape honey was 92.3%.This study would provide data support and reference for the classification and identification of rape honey,acacia honey and linden honey.

关 键 词：蜂蜜 品种鉴别 氨基酸 多元统计分析 

分 类 号：TS209[轻工技术与工程—食品科学]