生物胺降解菌Staphylococcus xylosus JCM 2418的特性研究及应用  

Characterization Research and Application of Biogenic Amine Degrading Bacterium Staphylococcus xylosus JCM 2418

作　　者：张杨 李歆宇 马新秀 毕景然[1] 张公亮[1] 侯红漫[1] ZHANG Yang;LI Xinyu;MA Xinxiu;BI Jingran;ZHANG Gongliang;HOU Hongman(School of Food Science and Technology,Dalian Polytechnic University,Dalian 116034,Liaoning,China)

机构地区：[1]大连工业大学食品学院,辽宁大连116034

出　　处：《食品研究与开发》2023年第20期191-198,共8页Food Research and Development

基　　金：国家重点基础研究发展计划项目(973计划)(2017YFC1600403)。

摘　　要：从鱼露中筛选获得一株具有生物胺降解能力的木糖葡萄球菌——Staphylococcus xylosus JCM 2418,对其安全性、发酵性能进行评价同时对生物胺降解相关酶展开分析,并将其接种至发酵食品中进行应用,监测发酵食品生物胺降解率以及风味的变化情况。结果表明,S.xylosus JCM 2418表现出弱耐药性,不具有溶血性,不产生生物膜;具有良好的耐盐性、蛋白酶和脂肪酶活性;生物胺降解相关酶为细胞膜上的胺氧化酶;应用在发酵食品中,可以有效控制生物胺的积累,同时改善发酵产品的风味,为提升发酵产品品质提供优良菌种。A strain of Staphylococcus xylosus(S.xylosus)JCM 2418 with the ability to degrade biogenic amines was screened from fish sauce,and its safety evaluation and fermentation characteristics were evaluated.The biogenic amine degrading enzyme was analyzed,and it was inoculated into fermented food for application.The changes in the biogenic amine degrading rate and flavor of fermented food were monitored.The results showed that S.xylosus JCM 2418 showed weak resistance,no hemolytic,and no biofilm production.It had good salt tolerance,as well as protease and lipase activities.The biogenic amine degrading enzyme was amine oxidase on the cell membrane.The application in fermented food effectively controlled the accumulation of biogenic amines,improved the flavor of fermented products,and provided good strains for improving the quality of fermented products.

关 键 词：Staphylococcus xylosus JCM 2418 安全性 发酵特性 胺氧化酶 生物胺 风味 

分 类 号：TS201.3[轻工技术与工程—食品科学]