高温发酵产木糖醇菌株的构建  

Construction of Xylitol Producing Strain by High Temperature Fermentation

作　　者：欧阳兆东 OUYANG Zhaodong(Panlong Market Supervision Administration of Kunming City,Kunming 650000,China)

机构地区：[1]昆明市盘龙区市场监督管理局检验检测中心,云南昆明650000

出　　处：《食品安全导刊》2023年第8期122-124,共3页China Food Safety Magazine

摘　　要：本文以高效木糖发酵菌株和耐高温菌株为基因组资源,应用基因组改组技术手段,成功构建出耐高温发酵产木糖醇的创新基因组,并获得了改造菌株F5-3。与亲本菌株相比,改组菌株在43℃条件下能够循环利用细胞发酵,其木糖醇转化率高达80%,生成速率达到3.61 g·(L·h)^(-1),表现出了相当高的生产性能和可持续性。s:This paper utilized efficient xylose-fermenting strains and high temperature-tolerant strains as genome resources,and successfully constructed an innovative genome for high temperature-tolerant xylose fermentation to produce xylitol using genome shuffling technology.A modified strain,F5-3,was obtained and showed a high yield of xylitol,generating up to 80%conversion rate and a maximum production rate of 3.61 g·(L·h)^(-1) at a temperature of 43℃for sustained cell fermentation.

关 键 词：木糖醇发酵 木糖醇检测 食品工业 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]