基于HS-SPME-GC-MS技术分析意大利葡萄果实香气  

Analysis on Aroma Components of Grape Italia by HS-SPME-GC-MS

作　　者：李凯[1] 商佳胤[1] 王学智 张娜[1] 王超霞 王丹[1] 张鹤[1] 田淑芬 LI Kai;SHANG Jiayin;WANG Xuezhi;ZHANG Na;WANG Chaoxia;WANG Dan;ZHANG He;TIAN Shufen(Forestry and Pomology Institute,Tianjin Academy of Agricultural Sciences,Tianjin 300384;Horticulture and Landscape Architecture College,Tianjin Agricultural College,Tianjin 300384,China)

机构地区：[1]天津市农业科学院林业果树研究所,天津300384 [2]天津农学院园艺园林学院,天津300384

出　　处：《东北农业科学》2023年第4期106-112,共7页Journal of Northeast Agricultural Sciences

基　　金：天津市林果现代农业产业技术体系项目(ITTFPRS2018005);国家现代农业产业技术体系建设专项资金项目(CARS-29)。

摘　　要：采用顶空固相微萃取(HS-SPME)及气相色谱-质谱技术(GC-MS),内标-标准曲线定量、气味活性值分析及果实香气轮廓分析等方法,对天津产区玫瑰香型葡萄品种意大利的果实香气成分进行分析,研究其风味特性。结果显示:共检测出43种香气化合物,主要成分为醇类、醛类、萜烯类和酯类化合物,其中以1-己醇、反式-2-己烯-1-醛、反式-2-己烯-1-醇、里那醇、乙酸乙酯和正己醛为主。其果实香气主要由12种活性呈香成分贡献,气味活性值由高到低依次为:里那醇、正己醛、反式-2-己烯-1-醛、1-辛烯-3-醇、苯乙醛、己酸乙酯、反式-2-己烯-1-醇、正庚醛、香茅醇、丁酸乙酯、1-己醇和香叶醇。香气轮廓直观地显示其果实香气主要由植物类、水果类、花卉类和脂肪类气味构成,4种气味系列强度较为均衡。A comprehensive analysis of the aroma components of Italia grapes,a muscat-flavor grapes grown in Tianjin,was conducted using headspace solid phase micro-extraction(HS-SPME),gas chromatography-mass spectrometry(GC-MS),along with methods such as internal standard-standard curve quantification,odor activity value analysis and aroma profile analysis.The results showed that a total of 43 aroma components with alcohols,aldehydes,terpenes,and esters being the major components.The main compounds identified included 1-hexanol,trans-2-hexen-1-al,trans-2-hexen-1-ol,linalool,ethyl acetate,and hexanal.Odor activity value results showed that aroma of grape Italia was mainly contributed by 12 kinds of active aroma components,including linalool,hexanal,trans-2-hexen-1-al,1-octen-3-ol,phenylacetaldehyde,ethyl hexanoate,trans-2-hexen-1-ol,heptanal,citronellol,ethyl butyrate,1-hexanol and geraniol(OAV from high to low).The visualized aroma profile showed that aroma of grape Italia was composed of plants,fruits,flowers and fats flavor,with a balanced intensity across the four odor series.

关 键 词：意大利葡萄 顶空固相微萃取 香气成分 气味活性值 香气轮廓 

分 类 号：S663.1[农业科学—果树学]