Rufibacter hautae NBS58-1胞外多糖的抗氧化活性及其在草莓保鲜中的应用  

Antioxidant activity of exopolysaccharide produced by Rufibacter hautae NBS58-1 and its application in strawberry preservation

作　　者：李春雨 屈建航[1] 周佳[1] 赵帅 罗宇[1] LI Chunyu;QU Jianhang;ZHOU Jia;ZHAO Shuai;LUO Yu(College of Biological Engineering,Henan University of Technology,Zhengzhou 450001,China)

机构地区：[1]河南工业大学生物工程学院,河南郑州450001

出　　处：《河南工业大学学报（自然科学版）》2023年第5期93-99,共7页Journal of Henan University of Technology：Natural Science Edition

基　　金：国家自然科学青年基金项目(42107139);河南工业大学创新基金支持计划专项(2021ZKCJ15);郑州市R&D专项经费补助科研项目(31310117)。

摘　　要：微生物胞外多糖具有良好的抗氧化能力,为了探究来自红杆菌Rufibacter hautae NBS58-1的胞外多糖的抗氧化活性及其在草莓保鲜中的应用,采用乙醇沉淀法制备菌株NBS58-1的胞外多糖(EPS-1),利用高效液相色谱和凝胶渗透色谱法分析其单糖组成和分子量,通过自由基清除试验分析其抗氧化活性,测定草莓的质量损失率、可溶性固形物含量、丙二醛含量和抗坏血酸含量以考察EPS-1的保鲜效果。结果表明:EPS-1由葡萄糖、甘露糖、核糖、半乳糖、阿拉伯糖、木糖、半乳糖醛酸、鼠李糖、岩藻糖和葡萄糖醛酸组成,摩尔比为80.33∶11∶2.84∶2.74∶1.79∶0.61∶0.26∶0.17∶0.15∶0.1,分子量为4652;EPS-1质量浓度为5 mg/mL时,对ABTS、DPPH和羟基自由基的清除率分别为(81.48±2.09)%、(41.47±0.37)%和(91.59±0.73)%,总还原力在700 nm处的吸光度为0.29;在草莓保鲜应用中,EPS-1可减少草莓的质量损失及丙二醛含量,延缓可溶性固形物降解和抗坏血酸氧化。由此可知,红杆菌Rufibacter hautae NBS58-1的胞外多糖EPS-1是一种杂多糖,具有自由基清除活性,可延长草莓保质期。Some microbial exopolysaccharides have efficient antioxidant capacity.In order to investigate the antioxidant activity of exopolysaccharides extracted from Rufibacter hautae NBS58-1 and study its function in strawberry preservation,the exopolysaccharide(EPS-1)from strain NBS58-1 was prepared by ethanol precipitation.The composition and molecular weight of the EPS-1 were analyzed by high performance liquid chromatography(HPLC)and gel permeation chromatography(GPC),the antioxidant activity of EPS-1 was explored by free radical scavenging experiments,and its application in strawberry preservation was explored.The results showed that the EPS-1 was composed of glucose,mannose,ribose,galactose,arabinose,xylose,galacturonic acid,rhamnose,fucose and glucuronic acid with a ratio of 80.33∶11∶2.84∶2.74∶1.79∶0.61∶0.26∶0.17∶0.15∶0.1,and the molecular weight was 4652.The results of free radical scavenging experiments showed that the antioxidant activity of EPS-1 was positively correlated with its mass concentration in the concentration range of 0-5 mg/mL.At the concentration of 5 mg/mL,the scavenging rates of ABTS,DPPH,and hydroxyl radical were(81.48±2.09)%,(41.47±0.37)%,and(91.59±0.73)%,respectively.The absorption value of total reducing power at 700 nm was 0.29.When EPS-1 was used as a coating solution for strawberries,the mass loss rate of strawberries was(55.58±0.89)%after 7 d,which was smaller than that of the control group.The content of SSC was(7.03±0.12)%,which was significantly higher than that in the control group.The MDA content was 4.07μmol/L,which was significantly lower than that of the control group.The content of Vc was(23.63±0.67)μg/g,which was significantly higher than that of the control group.These results showed that EPS-1 could reduce the weight loss and malonaldehyde content of strawberry,delay the degradation of soluble solids and Vc oxidation.Therefore,EPS-1 from R.hautae NBS58-1 is a heteropolysaccharide with free radical scavenging activity and can prolong the shelf life of

关 键 词：微生物 胞外多糖 抗氧化 草莓保鲜 

分 类 号：TS201.3[轻工技术与工程—食品科学]