响应面法优化超声辅助乙醇提取桑葚花青素工艺及稳定性分析  

Optimization of Process of Ultrasonic-Assisted Ethanol Extraction of Anthocyanins from Mulberry by Response Surface Methodology and Analysis of Their Stability

作　　者：邱菊[1] 陈昌琳 陈冰艺 何贵萍[1] 张佳琪[1] 吕远平[1] QIU Ju;CHEN Chang-lin;CHEN Bing-yi;HE Gui-ping;ZHANG Jia-qi;LYU Yuan-ping(College of Biomass Science and Engineering,Sichuan University,Chengdu 610065,China;Industrial Crop Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu 610300,China)

机构地区：[1]四川大学轻工科学与工程学院,成都610065 [2]四川省农业科学院经济作物育种栽培研究所,成都610300

出　　处：《中国调味品》2023年第11期176-182,共7页China Condiment

基　　金：四川省科技计划项目(重点研发项目)(2020YFN0149);四川省重点研发项目(省院省校科技合作)(2022YFSY0031)。

摘　　要：以桑果干为原料,利用单因素试验探究乙醇浓度、料液比、超声时间、功率和温度等因素对花青素得率的影响,采用响应面法拟合回归模型,优化超声辅助乙醇提取桑葚中花青素的工艺,并研究不同光照、温度对桑葚花青素提取液稳定性的影响。结果表明,最优提取工艺为乙醇浓度65.588%、时间26.222 min、超声功率181.730 W、温度46.125℃,试验操作下花青素实际得率为7.815 mg/g,与理论得率7.824 mg/g基本相符。稳定性试验结果显示,避光条件下花青素拥有更高的稳定性,温度在60℃以下有利于花青素的保存,温度高于60℃时,t 1/2降至2.5 d,k>1.15×10^(-2),花青素降解加速。超声辅助乙醇提取法简便易行、成本低且效率高,优化的工艺参数和稳定性研究有利于扩大花青素的高效利用。花青素适于低温、避光保存。With dried mulberry as the raw material,single factor test is used to explore the effects of ethanol concentration,solid-liquid ratio,ultrasonic time,power and temperature on the yield of anthocyanins.Response surface methodology is used to fit the regression model and optimize the process of ultrasonic-assisted ethanol extraction of anthocyanins from mulberry,and the effect of different light and temperature conditions on the stability of anthocyanin extract from mulberry is studied.The results show that the optimal extraction process is as follows:the ethanol concentration is 65.588%,time is 26.222 min,ultrasonic power is 181.730 W and temperature is 46.125℃.Under these conditions,the actual yield of anthocyanins is 7.815 mg/g,which is basically consistent with the theoretical yield of 7.824 mg/g.The stability test results show that anthocyanins have higher stability under dark conditions,and the temperature below 60℃is conducive to the preservation of anthocyanins,when the temperature is higher than 60℃,t 1/2 decreases to 2.5 d and k>1.15×10^(-2),which accelerates the degradation of anthocyanins.Ultrasonic-assisted ethanol extraction method is simple and feasible,with low cost and high efficiency.The optimized process parameters and stability study are conducive to expanding the efficient utilization of anthocyanins.Anthocyanins are suitable for storage under low-temperature and dark conditions.

关 键 词：桑葚 花青素 超声提取 响应面法 稳定性 

分 类 号：TS264.4[轻工技术与工程—发酵工程]