桑黄营养成分、活性成分及多糖性质分析  

Analysis of Nutritional Components,Active Components and Polysaccharide Properties of Phellinus igniarius

作　　者：刘锦程 弓志青[1] 杨鹏[3] 王延圣[1] 李永生[1] 杨正友[2] 王文亮[1] LIU Jincheng;GONG Zhiqing;YANG Peng;WANG Yansheng;LI Yongsheng;YANG Zhengyou;WANG Wenliang(Institute of Food&Nutrition Science and Technology,Shandong Academy of Agricultural Sciences,Jinan 250100,China;College of Life Science,Shandong Agricultural University,Taian 271018,China;Institute of Agricultural Resource and Environment,Shandong Academy of Agricultural Sciences,Jinan 250100,China)

机构地区：[1]山东省农业科学院农产品加工与营养研究所,山东济南250100 [2]山东农业大学生命科学学院,山东泰安271018 [3]山东省农业科学院农业资源与环境研究所,山东济南250100

出　　处：《食品工业科技》2023年第22期241-248,共8页Science and Technology of Food Industry

基　　金：山东省现代农业产业技术体系食用菌产后加工岗位专家项目(SDAIT-07-09);山东省自然科学基金青年项目(ZR2022QC239)。

摘　　要：为探寻不同桑黄子实体中营养、活性成分及抗氧化活性差异,本研究比较分析了5种桑黄的营养、活性成分含量及其多糖的抗氧化活性,以此为评价指标筛选优质桑黄子实体,采用rDNA ITS序列分析对目标桑黄进行鉴定,并对其多糖的抗氧化活性进行测定,通过紫外光谱、傅里叶变换红外光谱和高效液相色谱分析多糖性质。结果表明,供试桑黄在营养、活性成分含量及多糖抗氧化活性方面均存在较大差异;其中,桑黄S-4中粗纤维、粗蛋白、多糖、总酚、总黄酮、总三萜含量相对较高,粗脂肪和灰分含量相对较低,且S-4多糖对DPPH自由基、ABTS^(+)自由基和自由基O_(2)^(-)的清除能力均较好,半抑制浓度(IC_(50))分别为35.07、12.87、91.34μg/mL,对ABTS^(+)自由基的清除能力与抗坏血酸相当;将S-4作为目标桑黄,经鉴定,为杨树桑黄;紫外、红外光谱和单糖组成分析表明S-4多糖具有典型的多糖类特征吸收峰,是含有β-糖苷键和呋喃环的多糖,主要由葡萄糖、半乳糖、岩藻糖、葡萄糖醛酸、核糖和甘露糖组成。S-4的营养、活性成分含量相对丰富,其多糖具有良好的抗氧化能力,为优良桑黄子实体的筛选及多糖开发利用提供参考。In order to explore the differences of nutrition,active components and antioxidant activity in the fruiting bodies of different Phellinus igniarius,this study compared the nutritional and active ingredient contents of five species of Phellinus igniarius as well as their antioxidant activity of polysaccharides for the sake of obtaining high quality Phellinus igniarius fruiting bodies.The target Phellinus igniarius was identified by rDNA ITS sequence analysis and the antioxidant activity of its polysaccharide was determined.The properties of polysaccharides were analyzed by ultraviolet spectrum,Fourier transform infrared spectrum and high performance liquid chromatography.The results showed that significant differences in the nutrition,active ingredient content and polysaccharide antioxidant activity of different species were observed.The contents of crude fiber,crude protein,polysaccharide,total phenol,total flavone and total triterpene in S-4 were relatively high,while the contents of crude fat and ash were relatively low.S-4 polysaccharides demonstrated excellent DPPH radical,ABTS^(+)radical and O_(2)^(-) radical scavenging ability,and the half-inhibitory concentration(IC50)were 35.07,12.87 and 91.34μg/mL,respectively.The scavenging ability of S-4 polysaccharides against ABTS^(+)radical was equivalent to that of ascorbic acid.S-4 was used as the target Phellinus igniarius,and was identified as Sanghuangporus vaninii.Ultraviolet,infrared spectrum and monosaccharide composition analysis showed that S-4 polysaccharides had typical polysaccharide characteristic absorption peak,which was composed of glucose,galactose,fucose,glucuronic acid,ribose and mannose,containingβ-glycoside bond and furan ring.The S-4 was relatively rich in nutrients and active ingredients,and its polysaccharides had good antioxidant capacity,which would provide a reference for the screening of excellent Phellinus igniarius fruiting bodies and the development and utilization of polysaccharides.

关 键 词：桑黄 多糖 抗氧化能力 营养成分 单糖组成 

分 类 号：R931.6[医药卫生—生药学]