水解单宁酸脱除绿茶提取液中咖啡因的研究  

A Study of Hydrolyzed Tannins to Remove Caffeine from Green Tea Extract

作　　者：范玉文 聂珂楠 李婷 王玉丽 吴正奇 FAN Yuwen;NIE Kenan;LI Ting;WANG Yuli;WU Zhengqi(School of Bioengineering and Food,Hubei University of Technology,Wuhan 430068)

机构地区：[1]湖北工业大学生物工程与食品学院,武汉430068

出　　处：《食品工业》2023年第10期78-83,共6页The Food Industry

基　　金：国家自然科学基金(31871813)。

摘　　要：试验以绿茶提取液为原料,采用水解单宁酸脱除提取液中的咖啡因,为生产低咖啡因产品或者无咖啡因绿茶茶粉提供技术和理论的借鉴。以咖啡因残留率、表没食子儿茶素没食子酸酯(EGCG)保留率、EGCG与咖啡因的相对分离度为指标,以水解单宁酸溶液的添加比例、搅拌时间、温度、pH和浓缩比为因素,优化水解单宁酸脱除咖啡因的最佳条件。结果表明,最佳条件为绿茶提取液的浓缩比1/4、水解单宁酸溶液添加比例采用浓缩液体积的20%、搅拌时间20 min、温度35℃和pH 5.5。在此条件下,咖啡因脱除率为88%,残留率为12%, EGCG损失率为25%,保留率为75%,相对分离度为6.3。Tea was used as raw material to remove caffeine by hydrolyzed tannic acid from the extract,which provided technical and theoretical reference for the production of decaffeinated green tea powder or decaffeinated green tea powder.With the caffeine residue rate,the retention rate of epigallocatechingallate(EGCG),the relative separation degree of EGCG and caffeine as indexes,and with the proportion of hydrolyzed tannin solution added,stirring time,temperature,pH and concentration ratios as factors,the optimal condition for the removal of caffeine by hydrolyzed tannin solution was optimized.The results showed that the optimal condition was as follows:the concentration ratio of green tea extract 1/4,the addition ratio of hydrolyzed tannic acid solution 20% of the concentration volume,the stirring time 20 min,the temperature 35℃,and the pH 5.5.Under this condition,the caffeine removal rate was 88%,the residual rate was 12%,the loss rate of EGCG was 25%,the retention rate was 75%,and the relative resolution was 6.3.

关 键 词：绿茶 水解单宁酸 脱咖啡因 表没食子儿茶素没食子酸酯 相对分离度 

分 类 号：TS272[农业科学—茶叶生产加工]