儿茶素对阪崎肠杆菌的体外抑制作用  

Inhibitory effect of catechin on Enterobacter sakazakii in vitro

作　　者：龚国利[1] 胡乐梅 加欣宜 齐琦 GONG Guo-li;HU Le-mei;JIA Xin-yi;QI Qi(School of Food Science and Engineering,Shaanxi University of Science&Technology,Xi′an 710021,China)

机构地区：[1]陕西科技大学食品科学与工程学院,陕西西安710021

出　　处：《陕西科技大学学报》2023年第6期62-67,共6页Journal of Shaanxi University of Science & Technology

基　　金：陕西省教育厅专项科研计划项目(21JK0541);陕西省咸阳市秦创原科技创新专项项目(2021ZDZX-NY-0007)。

摘　　要：阪崎肠杆菌(Enterobacter sakazakii)是一种食源性致病菌,易引发坏死性肠炎、脑膜炎和婴儿败血症等多种疾病.儿茶素作为一种存在于茶叶等天然植物中的天然多酚类产物,具有良好的抗菌活性.本研究通过激光共聚焦扫描显微镜和场发射扫描电镜观察发现,儿茶素作用后E.sakazakii细胞膜发生破损,细胞表面粘连凹陷,出现孔洞;在1 MIC浓度作用下,E.sakazakii膜电位升高24.79%,胞内活性氧水平增加4.72倍,胞内ATP下降4.93倍,胞内pH下降45.63%.上述结果表明,儿茶素可通过破坏细胞膜完整性、改变细胞膜通透性来达到抑菌效果.该研究为儿茶素作为天然抗菌剂的应用奠定理论基础.Enterobacter sakazakii is a food-borne bacterium that causes diseases as diverse as necrotizing enteritis,meningitis and infantile sepsis.Catechin is a kind of natural polyphenol products existing in tea and other natural plants,which has good antibacterial activity.In this study,laser confocal scanning microscopy and field emission scanning electron microscopy observations showed that the cell membrane of E.sakazakii was damaged after the action of catechin,and the cell surface adhered and pitted with holes.Under 1 MIC concentration,E.sakazakii membrane potential increased by 24.79%,intracellular reactive oxygen species increased by 4.72 times,intracellular ATP decreased by 4.93 times,and intracellular pH decreased by 45.63%.These results indicate that catechin can achieve antibacterial effect by disrupting cell membrane integrity and changing cell membrane permeability.This study lays a theoretical foundation for the application of catechin as a natural antibacterial agent.

关 键 词：儿茶素 阪崎肠杆菌 抑菌活性 

分 类 号：TS201.3[轻工技术与工程—食品科学]