耐热型植物乳杆菌微胶囊粉剂  

Study on heat-resistant powder of Lactobacillus plantarum microcapsule

作　　者：刘艳霞 管军军[1] 崔耀明 LIU Yanxia;GUAN Junjun;CUI Yaoming(College of Biological Engineering,Henan University of Technology,Zhengzhou,Henan 450000,China)

机构地区：[1]河南工业大学生物工程学院,河南郑州450000

出　　处：《食品与机械》2023年第10期184-191,共8页Food and Machinery

基　　金：国家重点研发计划(编号:2021YFD1300300)。

摘　　要：目的:提高植物乳杆菌的耐热性。方法:在前期研究的微胶囊乳液中加入2-羟丙基-β-环糊精(2-HP-β-CD),通过喷雾干燥法制备植物乳杆菌微胶囊粉剂。通过响应面法,对不同参数进行优化。采用光学显微镜、电子显微镜对其形态进行观察,通过激光粒度分析仪及Zeta电位分析仪测定其粒径电位,利用分光光度计测定其吸光度变化,并采用烘箱加热的方式进行耐热性试验。结果:随2-HP-β-CD与乳液质量比、进料速率、进风温度、进风速率增加,存活率呈先升后降的趋势(P<0.05);在2-HP-β-CD与乳液质量比为3.13、进料速率为288 mL/h、进风速率为112 m^(3)/h、进风温度为118℃时,菌体存活率为(99.53±0.11)%,载菌量为1.64×10^(9)CFU/g;扫描电镜和显微结果显示微胶囊为中心凹陷的微粒,在水中呈球形分散,菌体聚集于内部,其水分散液吸光值1 h内变化不显著,Z-平均粒径为472.7 nm且粒径分布较集中,Zeta电位为-40.37 mV;微胶囊在130℃处理60 s,存活率为96.79%,与菌泥差异极其显著(P<0.01)。结论:该微胶囊粉剂具有较高的耐热性与较稳定的水分散性。Objective:This study aimed to improve the heat tolerance of Lactobacillus plantarum.Methods:On the basis of the previous research on the microcapsule emulsion,a kind of powder was prepared by spray drying with 2-hydroxypropyl-β-cyclodextrin(2-HP-β-CD).The response surface method is used to optimize different parameters.Its morphology was observed by light microscope and electron microscope,and the particle size was determined by laser particle size analyzer and Zeta potential analyzer;the absorbance change was measured by spectrophotometer,and heat resistance test was carried out by oven heating.Results:With the increase of the ratio of 2-HP-β-CD to emulsion mass,feed rate,inlet air temperature and inlet air rate,and the survival rate first increased and then decreased(P<0.05).By response surface methodology,the optimal parameters of spray drying were obtained as follows:the ratio of 2-HP-β-CD to emulsion mass 3.13,feed rate 288 mL/h,inlet air rate 112 m^(3)/h and the inlet air temperature 118℃.Under the control fo these conditions,the survival rate of bacteria was(99.53±0.11)%,and the bacterial load reached 1.64×10^(9)CFU/g.The scanning electron microscopy and microscopy results showed that the microcapsule was a central concave particle,spherically dispersed in water with the bacteria gathered inside.The absorbance value of the water dispersion was not found changing significantly within 1 h(P>0.05),and the average particle size was 472.7 nm with distribution concentrated and Zeta potential at-40.37 mV.The survival rate of microcapsules was 96.79%when treated at 130℃for 60 s,which was significantly different from the survival rate of bacterial sludge(P<0.01).Conclusion:The microcapsule powder has high heat resistance and stable water dispersibility.

关 键 词：植物乳杆菌 微胶囊粉剂 喷雾干燥 耐热性 存活率 

分 类 号：TS201.3[轻工技术与工程—食品科学]