低度白酒中白色絮状物与爽滑剂的检测  

Determination of white flocculent and slipping agent in low-alcohol Baijiu

作　　者：张智力 杨学军 李瑞芬 吴艳凤 王微山 ZHANG Zhili;YANG Xuejun;LI Ruifen;WU Yanfeng;WANG Weishan(National Inspection and Testing Center for Packaging Product(Jinan),Shandong Provincial Key Laboratory of Test Technology for Material Chemical Safety,Shandong Institute for Product Quality Inspection,Jinan 250102,China)

机构地区：[1]山东省产品质量检验研究院、山东省材料化学安全检测技术重点实验室国家包装产品质量检验检测中心(济南),山东济南250102

出　　处：《中国酿造》2023年第11期249-253,共5页China Brewing

基　　金：科技计划项目(2021MR11)。

摘　　要：该研究采用气相色谱-质谱(GC-MS)联用技术建立低度白酒中白色絮状物及爽滑剂的检测方法。白酒样品经-20℃处理24 h后,采用0.45μm滤膜过滤,收集白色絮状物后用3 mL异丙醇淋洗,对其淋洗液进行GC-MS分析,并对爽滑剂定量分析进行方法学考察。结果表明,低度白酒样品中检出了白色絮状物棕榈酸乙酯、亚油酸乙酯、油酸乙酯及爽滑剂(油酸酰胺、芥酸酰胺),爽滑剂在质量浓度0.5~50.0 mg/L范围内线性关系良好(相关系数R2>0.998),平均加标回收率为92%~98%,精密度试验结果相对标准偏差(RSD)为0.8%~2.3%,检出限均为0.2 mg/kg,定量限均为0.5 mg/kg。该方法前处理简便、灵敏度高、准确性高,可以用于低度白酒中白色絮状物的定性及爽滑剂(油酸酰胺)的定量分析。A method for the determination of white flocculent and slipping agent in low-alcohol Baijiu was established by GC-MS.After the Baijiu samples were treated at-20℃for 24 h,filtered by 0.45μm filter membrane,the white flocculent was collected and washed with 3 ml isopropyl alcohol,the eluent was analyzed by GC-MS,and the methodology for quantitative analysis of the slipping agent was investigated.The results showed that white flocculent ethyl palmitate,ethyl linoleate,ethyl oleate and slipping agent(oleic acid amide,erucamide)were detected in low-alcohol Baijiu samples.The linear relationship of the slipping agent was good(correlation coefficient R2>0.998)in the mass concentration range of 0.5-50.0 mg/L,the average recovery rates were 92%-98%,the relative standard deviations(RSD)of precision tests results were 0.8%-2.3%,the limit of detection both was 0.2 mg/kg,and the limit of quantitation both was 0.5 mg/kg.The method was simple,sensitive and accurate,and could be used for qualitative analysis of white flocculent and quantitative analysis of slipping agent(oleic acid amide)in low-alcohol Baijiu.

关 键 词：气相色谱-质谱联用 低度白酒 白色絮状物 爽滑剂 检测 

分 类 号：TS262.5[轻工技术与工程—发酵工程]