抗氧化肽在真空冷冻干燥过程中对乳双歧杆菌Probio-M8的保护作用  

Protective Effect of Antioxidant Peptides on Bifidobacterium animalis subsp.lactis Probio-M8 during Vacuum Freeze-Drying

作　　者：王昊乾 张静雯 杨雄洲 刘青轩 姚国强[1] 孙天松[1] Wang Haoqian;Zhang Jingwen;Yang Xiongzhou;Liu Qingxuan;Yao Guoqiang;Sun Tiansong(Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education,Key Laboratory of Dairy Products Processing,Ministry of Agriculture and Rural Affairs,Inner Mongolia Agricultural University,Hohhot 010018)

机构地区：[1]内蒙古农业大学乳品生物技术与工程教育部重点实验室、农业农村部奶制品加工重点实验室,呼和浩特010018

出　　处：《中国食品学报》2023年第10期32-40,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31771954)。

摘　　要：目的:探究乳清蛋白水解物经分离纯化获得的抗氧化肽HP3-2在真空冷冻干燥过程中对乳双歧杆菌Probio-M8的保护作用。方法:以10%海藻糖为基础冻干保护剂并添加5 mg/mL的乳清蛋白为对照组,以添加分离纯化的抗氧化肽HP3-2为试验组,分别与乳双歧杆菌Probio-M8按质量比1∶1.5混合,真空冷冻干燥后,使用平板计数法、流式细胞术、气相色谱质谱联用仪对乳双歧杆菌Probio-M8的活菌数、细胞膜脂肪酸组成、细胞膜流动性、细胞内外pH值、Na+浓度、细胞膜电位、细胞内ROS水平和脂质氧化产物丙二醛含量进行测定。结果:5 mg/mL抗氧化肽HP3-2能够提高乳双歧杆菌M8在真空冷冻干燥后的菌种存活率(80.40±3.34)%,显著高于含有乳清蛋白的对照组(64.69±2.47)%,同时还能够降低乳双歧杆菌Probio-M8胞内活性氧,使脂质氧化产物丙二醛的含量(2.14±0.06)nmol/mL显著低于WP组(2.30±0.05)nmol/mL和T组(2.33±0.05)nmol/mL,维持细胞膜电位和质子梯度,使细胞膜中的不饱和脂肪酸含量(51.49±1.26)显著高于WP组(39.96±1.57)和T组(33.33±0.62),从而提高细胞膜流动性。结论:抗氧化肽HP3-2能够降低真空冷冻干燥过程中氧化应激对乳双歧杆菌Probio-M8的细胞损伤。Antioxidant peptide 3-2 which is isolated and purified in whey protein hydrolysate for the protection of Bifidobacterium animalis subsp.lactis Probio-M8(B.lactis Probio-M8)during vacuum freeze-drying.Whey protein was added to a basal lyoprotectant containing 10%trehalose as a control group and antioxidant peptide 3-2 was added as an experimental group.After freeze-drying,the viable count,cell membrane structure,intracellular and extracellular pH,as well as intracellular ROS levels and malondialdehyde content of lipid oxidation products due to oxidative stress,were measured in Probio-M8.Results:Antioxidant peptide HP3-2(5 mg/mL)could improve the survival rate(80.40±3.34)% of Probio-M8 after freeze-drying,which was significantly higher than the control group of whey protein(64.69±2.47)%.It was also able to reduce the intracellular reactive oxygen species,which resulted in the content of malondialdehyde(2.14±0.06)nmol/mL,a product of lipid oxidation,to be significantly lower than WP group(2.30±0.05)nmol/mL and T group(2.33±0.05)nmol/mL.It also could maintain the cell membrane potential and proton gradient,and the unsaturated fatty acid of the cell membrane(51.49±1.26)was significantly higher than WP group(39.96±1.57)and T group(33.33±0.62),thus increasing cell membrane fluidity.Conclusion:Antioxidant peptide 3-2 can protect the Probio-M8 during freeze-drying.

关 键 词：抗氧化肽 冷冻干燥 乳双歧杆菌Probio-M8 细胞膜 

分 类 号：TS201.3[轻工技术与工程—食品科学]