1株高产生物膜发酵乳杆菌抗胁迫特性与全基因组分析  

Stress Resistance and Whole Genome Analysis of a Lactobacillus fermentum with High Biofilm Production

作　　者：张悦[1,2] 蒋柠 郑砚学 顾悦 王艳[1] 贺银凤 Zhang Yue;Jiang Ning;Zheng Yanxue;Gu Yue;Wang Yan;He Yinfeng(College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018;Inner Mongolia Institute of Quality and Standardization,Hohhot 010070)

机构地区：[1]内蒙古农业大学食品科学与工程学院,呼和浩特010018 [2]内蒙古自治区质量和标准化研究院,呼和浩特010070

出　　处：《中国食品学报》2023年第10期63-72,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31960467)。

摘　　要：为探究高产生物膜发酵乳杆菌733的抗性机理,测定其对模拟人工胃肠液、酸、胆盐、温度和盐胁迫的耐受性以及有害代谢产物的分泌情况,同时对其全基因组进行测序分析。结果显示,高产生物膜发酵乳杆菌733在模拟人工胃肠液中的存活率分别为(98.1±0.5)%和(98.1±1.2)%,存活率较高,且不产生有害代谢产物;不同胁迫环境均会抑制菌株生长,而在某些特定条件下会刺激菌株产生应激响应以保证菌株活力;发酵乳杆菌733在经历不同环境胁迫时,主要对trxA、perR、ctsR和argR基因产生影响,而这些基因调控菌株抗逆性的作用方式还有待研究。To explore the resistance mechanism of high biofilm production in Lactobacillus fermentum 733,its tolerance to simulated artificial gastrointestinal fluids,acid,bile salts,temperature,and salt stress,as well as the secretion of harmful metabolites,were determined.Additionally,a whole-genome sequencing analysis was conducted.The results showed that the survival rates of high biofilm production L.fermentum 733 in simulated artificial gastrointestinal fluids were(98.1±0.5)% and(98.1±1.2)%,respectively,indicating high survival rates,and the strain did not produce harmful metabolites.Growth of the strain was inhibited in different stress environments,while under specific conditions,it stimulated stress responses to ensure strain vitality.The L.fermentum 733 mainly affected the trxA,perR,ctsR and argR genes when experiencing different environmental stresses,however,how these genes regulate the stress resistance of strain remains to be further studied.

关 键 词：发酵乳杆菌 抗胁迫特性 全基因组 应激机制 

分 类 号：TS201.3[轻工技术与工程—食品科学]